大鼠腹膜巨噬细胞中自由基产生随年龄增长而减少。
Decrease in free-radical production with age in rat peritoneal macrophages.
作者信息
Alvarez E, Conde M, Machado A, Sobrino F, Santa Maria C
机构信息
Departamento de Bioquimica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, Spain.
出版信息
Biochem J. 1995 Dec 1;312 ( Pt 2)(Pt 2):555-60. doi: 10.1042/bj3120555.
Abstract
The respiratory-burst reaction has been studied in rat peritoneal macrophages of different ages (3, 12 and 24 months) using phorbol 12-myristate 13-acetate (PMA) to stimulate NADPH oxidase. Production of O2-. and H2O2 decreased with age (about 50 and 75% respectively); however, no difference in NADPH oxidase activity was found. NO. production was also reduced with age (40%). Furthermore, a progressive and significant decrease in the pentose phosphate flux was detected as a function of age in control and PMA-stimulated macrophages. The NADPH/NADP+ ratio decreased with age in control and PMA-stimulated macrophages. Glucose uptake was lower in middle-aged (12 months) and old (24 months) animals but no differences were found between these groups.
摘要
利用佛波醇12 -肉豆蔻酸酯13 -乙酸酯（PMA）刺激NADPH氧化酶，对不同年龄（3、12和24个月）大鼠腹膜巨噬细胞的呼吸爆发反应进行了研究。超氧阴离子（O2-）和过氧化氢（H2O2）的产生随年龄增长而减少（分别约为50%和75%）；然而，未发现NADPH氧化酶活性存在差异。一氧化氮（NO）的产生也随年龄增长而降低（40%）。此外，在对照和PMA刺激的巨噬细胞中，检测到磷酸戊糖通量随年龄增长而逐渐显著下降。在对照和PMA刺激的巨噬细胞中，NADPH/NADP+比值随年龄增长而降低。中年（12个月）和老年（24个月）动物的葡萄糖摄取较低，但这两组之间未发现差异。
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本文引用的文献
1
THE ESTIMATION OF THE OXIDIZED AND REDUCED FORMS OF THE NICOTINAMIDE NUCLEOTIDES.烟酰胺核苷酸氧化型和还原型的测定
Biochem J. 1965 Apr;95(1):161-6. doi: 10.1042/bj0950161.
2
Metabolic and functional changes in lymphocytes and macrophages as induced by ageing.
Physiol Behav. 1993 Apr;53(4):651-6. doi: 10.1016/0031-9384(93)90169-g.
3
Age-dependent modification of lipid composition and lipid structural order parameter of rat peritoneal macrophage membranes.
Mech Ageing Dev. 1993 Oct 1;71(1-2):1-12. doi: 10.1016/0047-6374(93)90030-u.
4
Involvement of free radicals in ageing: a consequence or cause of senescence.自由基与衰老的关系：衰老的结果还是原因。
Br Med Bull. 1993 Jul;49(3):653-67. doi: 10.1093/oxfordjournals.bmb.a072638.
5
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Biochim Biophys Acta. 1993 Nov 24;1179(3):247-52. doi: 10.1016/0167-4889(93)90079-5.
6
Parallel induction of nitric oxide and glucose-6-phosphate dehydrogenase in activated bone marrow derived macrophages.
Biochem Biophys Res Commun. 1993 Oct 15;196(1):342-7. doi: 10.1006/bbrc.1993.2254.
7
Effect of ageing on tissue levels of amino acids involved in the nitric oxide pathway in rat brain.衰老对大鼠脑内一氧化氮途径相关氨基酸组织水平的影响。
J Neural Transm Gen Sect. 1993;94(1):21-30. doi: 10.1007/BF01244980.
8
Nicotinamide inhibits nitric oxide synthase mRNA induction in activated macrophages.烟酰胺抑制活化巨噬细胞中一氧化氮合酶mRNA的诱导。
Biochem J. 1994 Jan 1;297 ( Pt 1)(Pt 1):53-8. doi: 10.1042/bj2970053.
9
Nitric oxide production from macrophages is regulated by arachidonic acid metabolites.巨噬细胞产生一氧化氮受花生四烯酸代谢产物的调节。
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