涩柿乙醇提取物和酸性乙醇提取物在过氧化氢刺激的Caco-2人结肠上皮细胞中的抗氧化活性。
Antioxidant activities of ethanolic and acidic ethanolic extracts of astringent persimmon in HO-stimulated Caco-2 human colonic epithelial cells.
作者信息
Kim Leeseon, Kim Yunyoung, Kwon Oran, Kim Ji Yeon
机构信息
1Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul, 01811 South Korea.
2Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, 03760 South Korea.
出版信息
Food Sci Biotechnol. 2017 Aug 18;26(4):1085-1091. doi: 10.1007/s10068-017-0156-5. eCollection 2017.
The aim of this study was to investigate the protective effects of ethanol (EAP) and acidic ethanol extracts (AEAP) of astringent persimmon fruits () against hydrogen peroxide (HO)-induced oxidative stress in Caco-2 epithelial cells. Levels of reactive oxygen species (ROS) and reduced glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT), as well as protein and DNA damage were measured. Treatment with HO induced oxidative stress in Caco-2 cells but had no effect on SOD activity. Both EAP and AEAP protected the cells against oxidative damage and exhibited similar effects on cellular ROS scavenging activity and protein carbonyl levels. However, AEAP was more potent than EAP in normalizing CAT activity and cytosolic GSH level, and in protecting against DNA damage. These results demonstrated that astringent persimmon fruit extracts were biologically active, and that the tannin-rich fraction (AEAP) possesses stronger protective activity against HO-induced oxidative stress in Caco-2 cells.
本研究旨在探讨涩柿果实的乙醇提取物（EAP）和酸性乙醇提取物（AEAP）对过氧化氢（H₂O₂）诱导的Caco-2上皮细胞氧化应激的保护作用。测定了活性氧（ROS）水平、还原型谷胱甘肽（GSH）水平、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及蛋白质和DNA损伤情况。用H₂O₂处理可诱导Caco-2细胞发生氧化应激，但对SOD活性无影响。EAP和AEAP均能保护细胞免受氧化损伤，且对细胞ROS清除活性和蛋白质羰基水平表现出相似的作用。然而，在使CAT活性和胞质GSH水平正常化以及防止DNA损伤方面，AEAP比EAP更有效。这些结果表明，涩柿果实提取物具有生物活性，且富含单宁的部分（AEAP）对H₂O₂诱导的Caco-2细胞氧化应激具有更强的保护活性。
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