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焦磷酸依赖性蔗糖代谢及其在蔗糖输入植物组织中被果糖2,6-二磷酸激活的过程。

Pyrophosphate-dependent sucrose metabolism and its activation by fructose 2,6-bisphosphate in sucrose importing plant tissues.

作者信息

Xu D P, Sung S J, Alvarez C A, Black C C

出版信息

Biochem Biophys Res Commun. 1986 Dec 15;141(2):440-5. doi: 10.1016/s0006-291x(86)80192-9.

DOI:10.1016/s0006-291x(86)80192-9
PMID:3026384
Abstract

In the presence of pyrophosphate and uridine diphosphate, sucrose was cleaved to form glucose 1-phosphate and fructose with soluble extracts from sucrose importing plant tissues. The glucose 1-phosphate then was converted through glycolysis to triose phosphates in a pyrophosphate-dependent pathway which was activated by fructose 2,6-bisphosphate. Much less activity, less than 5%, was found in sucrose exporting tissue extracts from the same plants. These findings suggest that imported sucrose is metabolized in the cytoplasm of plant tissues by utilizing pyrophosphate and that sucrose metabolism is partially regulated by fructose 2,6-bisphosphate.

摘要

在焦磷酸和尿苷二磷酸存在的情况下,利用蔗糖输入型植物组织的可溶性提取物可将蔗糖裂解,形成1-磷酸葡萄糖和果糖。然后,1-磷酸葡萄糖通过糖酵解在焦磷酸依赖途径中转化为磷酸丙糖,该途径由2,6-二磷酸果糖激活。在来自同一植物的蔗糖输出组织提取物中发现的活性要低得多,不到5%。这些发现表明,输入的蔗糖在植物组织的细胞质中通过利用焦磷酸进行代谢,并且蔗糖代谢部分受2,6-二磷酸果糖调节。

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