果糖2,6 -二磷酸及其硫代磷酸酯类似物。它们的水解以及对糖酵解和糖异生酶作用的比较。
Fructose 2,6-bisphosphate and its phosphorothioate analogue. Comparison of their hydrolysis and action on glycolytic and gluconeogenic enzymes.
作者信息
Rider M H, Kuntz D A, Hue L
机构信息
Institute of Cellular and Molecular Pathology, Louvain University Medical School, Brussels, Belgium.
出版信息
Biochem J. 1988 Jul 15;253(2):597-601. doi: 10.1042/bj2530597.
Abstract
Purified chicken liver 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase was phosphorylated either from fructose 2,6-bis[2-32P]phosphate or fructose 2-phosphoro[35S]thioate 6-phosphate. The turnover of the thiophosphorylated enzyme intermediate as well as the overall phosphatase reaction was four times faster than with authentic fructose 2,6-bisphosphate. Fructose 2-phosphorothioate 6-phosphate was 10-100-fold less potent than authentic fructose 2,6-bisphosphate in stimulating 6-phosphofructo-1-kinase and pyrophosphate:fructose 6-phosphate phosphotransferase, but about 10 times more potent in inhibiting fructose 1,6-bisphosphatase. The analogue was twice as effective as authentic fructose 2,6-bisphosphate in stimulating pyruvate kinase from trypanosomes.
摘要
纯化的鸡肝6-磷酸果糖-2-激酶/果糖2,6-二磷酸酶可由果糖2,6-双[2-³²P]磷酸或果糖2-磷酰[³⁵S]硫代酸6-磷酸进行磷酸化。硫代磷酸化酶中间体的周转以及整个磷酸酶反应比使用真实的果糖2,6-二磷酸快四倍。果糖2-磷酰硫代酸6-磷酸在刺激6-磷酸果糖-1-激酶和焦磷酸：果糖6-磷酸磷酸转移酶方面的效力比真实的果糖2,6-二磷酸低10-100倍，但在抑制果糖1,6-二磷酸酶方面的效力约高10倍。该类似物在刺激锥虫丙酮酸激酶方面的效果是真实果糖2,6-二磷酸的两倍。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Assays of protein kinase.蛋白激酶测定
Methods Enzymol. 1983;99:3-6. doi: 10.1016/0076-6879(83)99034-1.
3
Gluconeogenesis and related aspects of glycolysis.糖异生作用及糖酵解的相关方面。
Annu Rev Biochem. 1983;52:617-53. doi: 10.1146/annurev.bi.52.070183.003153.
4
A kinetic study of pyrophosphate: fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate.马铃薯块茎中焦磷酸：果糖-6-磷酸磷酸转移酶的动力学研究。用于果糖2,6-二磷酸的微量测定。
Eur J Biochem. 1982 Dec;129(1):191-5. doi: 10.1111/j.1432-1033.1982.tb07039.x.
5
The enzymology of 2,3-bisphosphoglycerate.2,3-二磷酸甘油酸的酶学
Adv Enzymol Relat Areas Mol Biol. 1980;51:211-53. doi: 10.1002/9780470122969.ch5.
6
Phosphoglycerate mutases: stereochemical course of the phosphoryl group transfers catalyzed by the cofactor-dependent enzyme from rabbit muscle and the cofactor-independent enzyme from wheat germ.磷酸甘油酸变位酶：由兔肌肉中依赖辅因子的酶和小麦胚芽中不依赖辅因子的酶所催化的磷酰基转移的立体化学过程。
Biochemistry. 1980 Feb 19;19(4):738-43. doi: 10.1021/bi00545a020.
7
Activation of rat heart phosphofructokinase-2 by insulin in vivo.胰岛素在体内对大鼠心脏磷酸果糖激酶-2的激活作用。
FEBS Lett. 1984 Oct 29;176(2):484-8. doi: 10.1016/0014-5793(84)81223-5.
8
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10
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