利用嗜鞣管囊酵母发酵甘蔗渣水解液中的己糖和戊糖生产乙醇。
Fermentation of hexoses and pentoses from sugarcane bagasse hydrolysates into ethanol by Spathaspora hagerdaliae.
机构信息
Enzymes and Biomass Laboratory, Biotechnology Institute, University of Caxias do Sul, Rua Francisco Getúlio Vargas, 1130, Caxias do Sul, RS, 95070-560, Brazil.
Biological Science Institute, Federal University of Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
出版信息
Bioprocess Biosyst Eng. 2019 Jan;42(1):83-92. doi: 10.1007/s00449-018-2016-y. Epub 2018 Sep 27.
The present study evaluated 13 strains of yeast for ethanol and xylitol production from xylose. Among them, Spathaspora hagerdaliae UFMG-CM-Y303 produced ethanol yields (Y) of 0.25 g g and 0.39 g g under aerobic and microaerophilic conditions, respectively, from a mixture of glucose and xylose in flasks. A pH of 5.0 and an inoculum of 3.0 × 10 cells mLr resulted in the highest ethanol yields. These conditions were tested in a bioreactor for fermenting a medium containing an enzymatic hydrolysate of sugarcane bagasse with 15.5 g L of glucose and 3 g L of xylose, and achieved a Y of 0.47 g g, in relation to total available sugar. These results suggest that S. hagerdaliae UFMG-CM-Y303 has potential for use in second-generation ethanol studies.
本研究评估了 13 株酵母菌株从木糖生产乙醇和木糖醇的能力。其中，Spathaspora hagerdaliae UFMG-CM-Y303 在有氧和微氧条件下从葡萄糖和木糖混合物的摇瓶中分别产生 0.25 和 0.39 g g 的乙醇产率（Y）。pH 值为 5.0 和接种量为 3.0×10 个细胞 mLr 时，可获得最高的乙醇产率。这些条件在含有甘蔗渣酶解物的培养基中进行了生物反应器发酵试验，该培养基中含有 15.5 g L 的葡萄糖和 3 g L 的木糖，相对于总可用糖，乙醇产率达到了 0.47 g g。这些结果表明，Spathaspora hagerdaliae UFMG-CM-Y303 具有用于第二代乙醇研究的潜力。
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