Cadete R M, Melo-Cheab M A, Dussán K J, Rodrigues R C L B, da Silva S S, Gomes F C O, Rosa C A
Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, Brazil.
J Appl Microbiol. 2017 Nov;123(5):1203-1213. doi: 10.1111/jam.13559. Epub 2017 Sep 11.
This study aimed to evaluate new d-xylose-fermenting yeasts from Brazilian ecosystems for the production of second-generation ethanol.
d-xylose-fermenting yeasts isolated from rotting wood and wood-boring insects were identified as the species Scheffersomyces parashehatae, Scheffersomyces illinoinensis, Spathaspora arborariae and Wickerhamomyces rabaulensis. Among the yeasts tested, those of Sc. parashehatae exhibited the highest ethanol production when cultivated on complex medium (Y = 0·437 g g ). Sheffersomyces illinoinensis and Sp. arborariae showed similar ethanol production in this assay (Y up to 0·295 g g ). In contrast, in sugarcane bagasse hemicellulosic hydrolysate, Sc. parashehatae and Sc. illinoinensis exhibited similar ethanol production (Y up to 0·254 g g ), whereas Sp. arborariae showed the lowest results (peak Y = 0·160 g g ). Wickerhamomyces rabaulensis exhibited a remarkable xylitol production (Y = 0·681 g g ), but producing low levels of ethanol (Y = 0·042 g g ).
The novel d-xylose-fermenting yeasts showed promising metabolic characteristics for use in fermentation processes for second-generation ethanol production, highlighting the importance of bioprospecting research of micro-organisms for biotechnological applications.
This study widens the scope for future researches that may examine the native yeasts presented, as limited studies have investigated these species previously.
本研究旨在评估从巴西生态系统中分离出的新型发酵木糖酵母用于生产第二代乙醇的能力。
从腐烂木材和蛀木昆虫中分离出的发酵木糖酵母被鉴定为拟舍夫酵母属（Scheffersomyces parashehatae）、伊利诺伊舍夫酵母（Scheffersomyces illinoinensis）、树状嗜孢酵母（Spathaspora arborariae）和拉包尔威克汉姆酵母（Wickerhamomyces rabaulensis）。在所测试的酵母中，拟舍夫酵母属在复合培养基上培养时乙醇产量最高（Y = 0·437 g/g）。伊利诺伊舍夫酵母和树状嗜孢酵母在该试验中显示出相似的乙醇产量（Y高达0·295 g/g）。相比之下，在甘蔗渣半纤维素水解物中，拟舍夫酵母属和伊利诺伊舍夫酵母的乙醇产量相似（Y高达0·254 g/g），而树状嗜孢酵母的结果最低（峰值Y = 0·160 g/g）。拉包尔威克汉姆酵母木糖醇产量显著（Y = 0·681 g/g），但乙醇产量较低（Y = 0·042 g/g）。
新型发酵木糖酵母在第二代乙醇生产的发酵过程中显示出有前景的代谢特性，突出了微生物生物勘探研究在生物技术应用中的重要性。
本研究拓宽了未来研究的范围，未来研究可能会考察所呈现的本地酵母，因为此前对这些物种的研究有限。
