Thomas M J, Shirley P S, Hedrick C C, DeChatelet L R
Biochemistry. 1986 Dec 2;25(24):8042-8. doi: 10.1021/bi00372a037.
Human polymorphonuclear leukocytes produce large quantities of superoxide when they attack and kill bacteria. However, superoxide is a weak oxidizing and reducing agent, and other more reactive oxygen species derived from reactions of superoxide are suggested to participate in the killing processes. To test the hypothesis that a reactive free radical or singlet oxygen is involved in bactericidal activity, human polymorphonuclear leukocytes were exposed to phagocytozable particles containing lipids that contain the easily autoxidized 1,4-diene moiety. After incubation the preparations were extracted and the extracts reduced with NaBH4 to convert hydroperoxides to stable alcohols. Using gas chromatography/mass spectrometry to analyze the extracts, we were unable to detect products unless iron salts were added to the medium. The products obtained by extraction are those that would be expected if both free radical chain autoxidation and 1O2 oxidation were taking place. In summary, we find that polymorphonuclear leukocytes do not cause peroxidation, implying that formation of strongly oxidizing free radicals is not an intrinsic property of the leukocyte. Added iron catalyzes peroxidation by activated leukocytes yielding an unusual distribution of hydroxylated products.
人类多形核白细胞在攻击和杀死细菌时会产生大量超氧化物。然而，超氧化物是一种弱氧化剂和还原剂，超氧化物反应衍生的其他更具活性的氧物种被认为参与了杀伤过程。为了验证活性自由基或单线态氧参与杀菌活性的假设，将人类多形核白细胞暴露于含有易自氧化的1,4 - 二烯部分的脂质的可吞噬颗粒中。孵育后，提取制剂并用硼氢化钠还原提取物，将氢过氧化物转化为稳定的醇。使用气相色谱/质谱分析提取物，除非向培养基中添加铁盐，否则我们无法检测到产物。通过提取获得的产物是如果同时发生自由基链式自氧化和单线态氧氧化所预期的产物。总之，我们发现多形核白细胞不会引起过氧化，这意味着强氧化性自由基的形成不是白细胞的固有特性。添加的铁通过活化的白细胞催化过氧化，产生异常分布的羟基化产物。
