Clifford D P, Repine J E
Methods Enzymol. 1984;105:393-8. doi: 10.1016/s0076-6879(84)05054-0.
O2 radicals are important in health and disease. The most commonly used ways of identifying O2 radicals in PMN are described above. Several shortcomings exist in these methods reflecting the unusual, complex nature of O2 radical biochemistry. Some general principles include (1) O2 radicals are very short-lived, reacting with many other compounds and each other quickly. (2) There are no highly specific assays for O2 radicals. (3) Highly specific scavengers of O2 radicals also do not exist. (4) No methods have been found to detect and quantitate O2 radicals in vivo. (5) Solubility and membrane permeability of various scavengers and/or test reagents may affect the measurement of O2 radicals in PMN and other biological systems. In general, the best approach to measurement of O2 radicals involves using the best assay available and showing that the reaction is inhibited by scavengers in proportion to their reactivity with the specific O2 radical being assayed.
氧自由基在健康和疾病中都很重要。上述介绍了在中性粒细胞中识别氧自由基最常用的方法。这些方法存在一些缺点，反映出氧自由基生物化学性质的独特性和复杂性。一些一般原则包括：（1）氧自由基寿命极短，会迅速与许多其他化合物相互反应。（2）不存在针对氧自由基的高度特异性检测方法。（3）也没有氧自由基的高度特异性清除剂。（4）尚未发现体内检测和定量氧自由基的方法。（5）各种清除剂和/或测试试剂的溶解度和膜通透性可能会影响中性粒细胞及其他生物系统中氧自由基的测量。一般来说，测量氧自由基的最佳方法是使用现有的最佳检测方法，并证明该反应被清除剂抑制的程度与它们对所检测的特定氧自由基的反应活性成正比。
