甜菜根汁补充对野百合碱诱导的大鼠肺动脉高压的影响。

Effects of Beet Juice Supplementation on Monocrotaline-Induced Pulmonary Hypertension in Rats.

机构信息

Department of Pharmacology, Kanazawa Medical University, Kahoku, Ishikawa, Japan.

Laboratory of Molecular and Pathological Pharmacology, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan.

出版信息

Am J Hypertens. 2019 Jan 15;32(2):216-222. doi: 10.1093/ajh/hpy144.

DOI:10.1093/ajh/hpy144

PMID:30265283

Abstract

BACKGROUND

Recently, attention has been focused on the cardiovascular protective effects of beet juice (BJ) with high amounts of nitrate. In this study, we examined the effect of BJ supplementation in a rat model of monocrotaline (MCT)-induced pulmonary hypertension (PH).

METHODS

MCT (60 mg/kg) was subcutaneously administered to rats, and BJ (prepared by dissolving BJ powder at a concentration of 1 g/l or 10 g/l in drinking water) supplementation was started from the day of, 1 week before, and 2 weeks after MCT injection. Saline-injected rats given drinking water were used as controls.

RESULTS

Low-dose BJ supplementation starting from the day of MCT injection exerted protective effects on the MCT-induced elevation of right ventricular systolic pressure, right ventricular hypertrophy, and pulmonary arterial remodeling, without causing a significant increase in plasma nitrite plus nitrate (NOx) levels. On the other hand, such beneficial effects were not observed with high-dose BJ supplementation, although the NOx levels were slightly higher than those in the low-dose group. In addition, low-dose BJ supplementation starting from 1 week before MCT injection did not improve PH symptoms, as described above. Furthermore, low-dose BJ supplementation starting from 2 weeks after MCT injection was ineffective against functional and morphological alterations in pulmonary circulation associated with MCT-induced PH.

CONCLUSIONS

Habitual ingestion of a suitable amount of BJ could be a potential option for preventing PH. However, beneficial effects cannot be expected when PH has developed to some degree.

摘要

背景

最近，人们关注富含硝酸盐的甜菜汁（BJ）对心血管的保护作用。在这项研究中，我们研究了 BJ 补充对野百合碱（MCT）诱导的肺动脉高压（PH）大鼠模型的影响。

方法

将 MCT（60mg/kg）皮下注射到大鼠中，从 MCT 注射前 1 周开始至 MCT 注射后 2 周，通过饮用含 1g/L 或 10g/L BJ 粉末的水进行 BJ 补充。给予生理盐水注射的大鼠饮用普通水作为对照。

结果

低剂量 BJ 补充（从 MCT 注射当天开始）对 MCT 诱导的右心室收缩压升高、右心室肥厚和肺血管重塑具有保护作用，而不会导致血浆亚硝酸盐加硝酸盐（NOx）水平显著升高。另一方面，高剂量 BJ 补充没有观察到这种有益作用，尽管 NOx 水平略高于低剂量组。此外，从 MCT 注射前 1 周开始的低剂量 BJ 补充并没有改善上述 PH 症状。此外，从 MCT 注射后 2 周开始的低剂量 BJ 补充对 MCT 诱导的 PH 相关肺循环功能和形态改变无效。