Leite Luciano Bernardes, Soares Leôncio Lopes, Guimarães-Ervilha Luiz Otávio, Costa Sebastião Felipe Ferreira, Generoso Sara Caco Dos Lúcio, Xavier Mirielly Alexia Miranda, Iasbik-Lima Thainá, Oliveira Leandro Licursi de, Della Lucia Ceres Mattos, Bianchi Sara Elis, Bassani Valquíria Linck, Herter Flavio Gilberto, Turck Patrick, da Rosa Araujo Alex Sander, Forte Pedro, Reis Emily Correna Carlo, Machado-Neves Mariana, José Natali Antônio
Exercise Biology Laboratory, Department of Physical Education, Federal University of Viçosa, Viçosa 36570-900, MG, Brazil.
Department of Sports, Instituto Politécnico de Bragança, 5300-253 Bragança, Portugal.
Nutrients. 2025 Mar 26;17(7):1145. doi: 10.3390/nu17071145.
To investigate whether the regular administration of blueberry extract and low-intensity resistance exercise training (RT), either alone or in combination, during the development of monocrotaline (MCT)-induced severe pulmonary arterial hypertension (PAH) in rats protect the left ventricle (LV) from redox dysregulation and pathological remodeling.
Groups of seven male Wistar rats were formed for the experiment: sedentary control; sedentary hypertensive; sedentary hypertensive blueberry; exercise hypertensive; and exercise hypertensive blueberry. PAH was experimentally induced through a single intraperitoneal administration of MCT at a dose of 60 mg/kg. One day after injection, the blueberry groups started receiving a daily dose of blueberry extract (100 mg/kg) by gavage, while the exercise groups initiated a three-week program of RT (ladder climbing; 15 climbs carrying 60% of maximum load; one session/day; 5 times/week). Echocardiographic evaluations were conducted 23 days after injection, and the rats were euthanized the next day to harvest LV tissue.
Separately, blueberry extract and RT mitigated augments in pulmonary artery resistance, LV tissue redox dysregulation (i.e., increased PC levels) and detrimental remodeling (i.e., reduced inflammation), and reductions in ejection fraction (EF) and fractional shortening (FS) caused by PAH. The combination of treatments prevented reductions in EF and FS, along with the development of a D-shaped LV.
blueberry extract and moderate-intensity resistance training administered during the development of MCT-induced severe PAH in rats prevented LV redox dysregulation and pathological remodeling, thereby preserving its function.
研究在大鼠因注射野百合碱（MCT）诱导严重肺动脉高压（PAH）的过程中，单独或联合定期给予蓝莓提取物及低强度抗阻运动训练（RT）是否能保护左心室（LV）免受氧化还原失调和病理重塑的影响。
将雄性Wistar大鼠分成每组7只的几组进行实验：久坐对照组；久坐高血压组；久坐高血压蓝莓组；运动高血压组；运动高血压蓝莓组。通过腹腔注射60mg/kg剂量的MCT来实验性诱导PAH。注射后一天，蓝莓组开始通过灌胃每日给予一剂蓝莓提取物（100mg/kg），而运动组开始为期三周的RT计划（爬梯；携带60%最大负荷攀爬15次；每天一次；每周5次）。在注射后23天进行超声心动图评估，次日对大鼠实施安乐死以获取LV组织。
单独来看，蓝莓提取物和RT减轻了PAH引起的肺动脉阻力增加、LV组织氧化还原失调（即PC水平升高）和有害重塑（即炎症减轻），以及射血分数（EF）和缩短分数（FS）的降低。联合治疗可防止EF和FS降低以及D形LV的形成。
在大鼠因MCT诱导严重PAH的过程中给予蓝莓提取物和中等强度抗阻训练可防止LV氧化还原失调和病理重塑，从而保留其功能。
