Klumpp T
Ann Biol Clin (Paris). 1977;35(3):221-6.
The antitrypsic capacity of serum is in good respect with the level of active alpha1-antitrypsin in serum. Its determination can be improved by a wholly automated procedure with a centrifugal analyser Gemsaec, which allows the measure of the reaction rate of the hydrolysis of a synthetic substrate (BAPNA) for trypsin. After mixing the plasma with bovine trypsin, the residual trypsic activity is measured. By this procedure it is easy to disclose rapidly the physiopathologic changes of proteinase inhibitors of the plasma, and these of the alpha1-antitrysin especially. Besides this technique can be used to study the proteinase inhibitors in other biological fluids like pleural fluid, ascites, and joint fluid.
血清的抗胰蛋白酶能力与血清中活性α1-抗胰蛋白酶水平密切相关。使用离心分析仪Gemsaec的全自动化程序可以改进其测定方法，该程序能够测量胰蛋白酶对合成底物（BAPNA）水解反应的速率。将血浆与牛胰蛋白酶混合后，测量残余的胰蛋白酶活性。通过该程序，可以轻松快速地揭示血浆中蛋白酶抑制剂的生理病理变化，尤其是α1-抗胰蛋白酶的变化。此外，该技术还可用于研究其他生物体液如胸水、腹水和关节液中的蛋白酶抑制剂。
