Qin N Y, Yang F Q, Wang Y T, Li S P
Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China.
J Pharm Biomed Anal. 2007 Jan 17;43(2):486-92. doi: 10.1016/j.jpba.2006.07.034. Epub 2006 Aug 23.
Curcuma longa (Zingiberaceae) is a native plant of southern Asia and is cultivated extensively throughout the warmer parts of the world. Jianghuang and Yujin are rhizome and tuberous root of C. longa, respectively, which were traditionally used as two Chinese medicines. In this paper, pressurized liquid extraction (PLE) and gas chromatography-mass spectrometry (GC-MS) were developed for quantitative determination/estimation of eight characteristic compounds including beta-caryophyllene, ar-curcumene, zingiberene, beta-bisabolene, beta-sesquiphellandrenendrene, ar-turmerone, alpha-turmerone and beta-turmerone in Jianghuang and Yujin. A HP-5MS capillary column (30 m x 0.25 mm i.d.) coated with 0.25 microm film 5% phenyl methyl siloxane was used for separation and selected ion monitoring (SIM) method was used for quantitation. Hierarchical cluster analysis based on characteristics of eight identified peaks in GC-MS profiles showed that 10 samples were divided into two main clusters, Jianghuang and Yujin, respectively. Four components such as ar-curcumene, ar-turmerone, alpha-turmerone and beta-turmerone were optimized as markers for quality control of rhizome (Jianghuang) and tuberous root (Yujin), which are two traditional Chinese medicines, from Curcuma longa.
姜黄（姜科）是一种原产于亚洲南部的植物，在世界较温暖的地区广泛种植。姜黄和郁金分别是姜黄的根茎和块根，传统上用作两种中药。本文采用加压液体萃取（PLE）和气相色谱 - 质谱联用（GC-MS）技术，对姜黄和郁金中包括β-石竹烯、莪术烯、姜烯、β-红没药烯、β-倍半水芹烯、莪术酮、α-莪术酮和β-莪术酮在内的8种特征性化合物进行定量测定/估算。采用涂覆有0.25μm膜厚5%苯基甲基硅氧烷的HP-5MS毛细管柱（30 m×0.25 mm内径）进行分离，并采用选择离子监测（SIM）法进行定量分析。基于GC-MS图谱中8个已鉴定峰的特征进行的层次聚类分析表明，10个样品分别分为两个主要类别，即姜黄和郁金。莪术烯、莪术酮、α-莪术酮和β-莪术酮这四种成分被优化为来自姜黄的两种传统中药（根茎（姜黄）和块根（郁金））质量控制的标志物。
