Intelligent Fingerprinting Ltd., 14-17 Evolution Business Park, Milton Road, Impington, Cambridge, UK.
LGC Ltd., Newmarket Road, Fordham, Cambridgeshire, UK.
J Anal Toxicol. 2019 Mar 1;43(2):88-95. doi: 10.1093/jat/bky068.
Here, we describe the use of a fluorescence based lateral flow competition assay for the screening of four classes of drugs, viz, Δ9-tetrahydrocannabinol (THC), cocaine (through the detection of benzoylecgonine, BZE), opiates (through the detection of morphine, MOR) and amphetamine (AMP) present in the sweat of a fingerprint. The Drug Screening Cartridge was specifically developed for fingerprint sample collection and analysis. For this study, the cut-offs were set at: 190, 90, 68 and 80 pg/fingerprint for THC, BZE, MOR and AMP, respectively. Working with three UK coroners, the Drug Screening Cartridge, together with its fluorescence reader, was applied to the detection of drugs in the sweat of a fingerprint from deceased individuals. The study shows that there was sufficient sweat present on the fingertips to enable analysis and that the Drug Screening Cartridge could detect the presence, or absence, of each drug. The presence of the drugs was confirmed using LC-MS-MS analysis of a second fingerprint sample collected simultaneously. Excellent correlation was achieved between the results obtained from the Drug Screening Cartridge and the LC-MS-MS analysis of the fingerprint samples obtained from 75 individuals. The accuracy of the results was: 99% for THC; 95% for BZE; 96% for MOR and 93% for AMP. The results obtained using the Drug Screening Cartridge were also compared to toxicological analysis of blood and urine samples with good correlation. The accuracy of the results between the Drug Screening Cartridge and blood was: 96%, 92%, 88% and 97% for THC, BZE, MOR and AMP, respectively. The comparison with urine showed an accuracy ranging between 86% and 92%. This fingerprint sample method has a collection time of just 5 s and a total analysis time of <10 mins. These results show that the lateral flow Drug Screening Cartridge is an excellent screening test to provide information on drug use from the sweat in a single fingerprint sample.
在这里，我们描述了一种基于荧光的侧向流动竞争测定法，用于筛选四类药物，即Δ9-四氢大麻酚（THC）、可卡因（通过检测苯甲酰基古柯碱，BZE）、阿片类药物（通过检测吗啡，MOR）和安非他命（AMP），这些药物存在于指纹的汗液中。药物筛选试剂盒是专门为指纹样本采集和分析而开发的。在这项研究中，截止值分别设定为：THC、BZE、MOR 和 AMP 的 190、90、68 和 80pg/指纹。与三位英国验尸官合作，药物筛选试剂盒及其荧光阅读器应用于检测从死者指纹中提取的汗液中的药物。研究表明，指尖上有足够的汗水进行分析，并且药物筛选试剂盒可以检测到每种药物的存在或不存在。使用同时收集的第二份指纹样本的 LC-MS-MS 分析确认了药物的存在。从 75 个人获得的指纹样本中，药物筛选试剂盒的结果与 LC-MS-MS 分析之间获得了极好的相关性。结果的准确性为：THC 为 99%；BZE 为 95%；MOR 为 96%；AMP 为 93%。使用药物筛选试剂盒获得的结果也与血液和尿液样本的毒理学分析进行了比较，相关性良好。药物筛选试剂盒与血液结果的准确性分别为：THC、BZE、MOR 和 AMP 的 96%、92%、88%和 97%。与尿液的比较显示，准确性在 86%至 92%之间。这种指纹样本采集方法仅需 5 秒，总分析时间<10 分钟。这些结果表明，侧向流动药物筛选试剂盒是一种极好的筛选测试，可以从单个指纹样本中的汗液中提供药物使用信息。
