Scherer Juliana Nichterwitz, Fiorentin Taís Regina, Sousa Tanara Rosangela Vieira, Limberger Renata Pereira, Pechansky Flavio
Center for Drug and Alcohol Research, Collaborating Center on Alcohol and Drugs - HCPA/SENAD, Hospital de Clínicas de Porto Alegre, Federal University of Rio Grande do Sul, Rua Professor Álvaro Alvim, 400, 90420-020 Porto Alegre, RS, Brazil.
Postgraduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Av. Ipiranga, 2752, 90610-000 Porto Alegre, RS, Brazil.
J Anal Toxicol. 2017 Jun 1;41(5):392-398. doi: 10.1093/jat/bkx018.
The use of point-of-collection testing (POCT) devices for drugs of abuse in oral fluid (OF) is an advantageous tool that has been used for different purposes-particularly traffic enforcement. However, even with the widespread report of cocaine consumption, the reliability of POCT devices has been reported in different magnitudes. This study evaluated the reliability of two POCT devices for the detection of cocaine in OF samples of 110 cocaine users: (i) the DDS2™ (cutoff = 30 ng/mL) and (ii) the Multi-Drugs Multi-Line-Twist Screen Test Device™ (MDML) (cutoff = 20 ng/mL). Results of the screening tests were compared with a Liquid Chromatography-Mass Spectrometry (LC-MS) assay. Sensitivity, specificity and accuracy of DDS2™ were 100, 77.77 and 80% when compared with LC-MS with a cutoff of 30 ng/mL, and 88.89, 89.15 and 89.09% with a cutoff of 10 ng/mL. The MDML™ device achieved sensitivity, specificity and accuracy of 100, 65.6 and 70.9% when compared with LC-MS with a cutoff of 20 ng/mL, and 92.6, 71.1 and 76.6% with a cutoff of 10 ng/mL. When compared with a 10 ng/mL cutoff, the DDS2™ achieved reliability parameters higher than 80%. On the other hand, the MDML™ device did not achieve the minimal recommendation of 80% for all parameters at the same time. Taking into consideration the reliability results showed here, the authors believe that the use of these POCT devices seems to be suitable for cocaine detection in forensic tests only if all positive specimens are further confirmed by a validated method.
使用即时检测（POCT）设备检测口腔液（OF）中的滥用药物是一种具有优势的工具，已被用于不同目的，尤其是交通执法。然而，即便有关于可卡因消费的广泛报道，但POCT设备的可靠性却有不同程度的报道。本研究评估了两种POCT设备在110名可卡因使用者的OF样本中检测可卡因的可靠性：（i）DDS2™（临界值 = 30 ng/mL）和（ii）多药物多线扭转筛查测试设备™（MDML）（临界值 = 20 ng/mL）。将筛查测试结果与液相色谱 - 质谱联用（LC - MS）分析进行比较。与临界值为30 ng/mL的LC - MS相比，DDS2™的灵敏度、特异性和准确性分别为100%、77.77%和80%；与临界值为10 ng/mL的LC - MS相比，分别为88.89%、89.15%和89.09%。与临界值为20 ng/mL的LC - MS相比，MDML™设备的灵敏度、特异性和准确性分别为100%、65.6%和70.9%；与临界值为10 ng/mL的LC - MS相比，分别为92.6%、71.1%和76.6%。与临界值为10 ng/mL相比，DDS2™的可靠性参数高于80%。另一方面，MDML™设备并未同时达到所有参数80%的最低推荐标准。考虑到此处显示的可靠性结果，作者认为仅当所有阳性样本通过经过验证的方法进一步确认时，使用这些POCT设备似乎才适用于法医检测中的可卡因检测。
