口腔疣状癌中的人乳头瘤病毒2型DNA

Human papillomavirus type 2 DNA in oral verrucous carcinoma.

作者信息

Adler-Storthz K, Newland J R, Tessin B A, Yeudall W A, Shillitoe E J

出版信息

J Oral Pathol. 1986 Oct;15(9):472-5. doi: 10.1111/j.1600-0714.1986.tb00661.x.

DOI:10.1111/j.1600-0714.1986.tb00661.x

Abstract

Tissues from patients with oral verrucous carcinoma were examined for the presence of human papillomavirus (HPV). The tissues were stained for the presence of the type common papillomavirus antigen by immunohistochemical staining and the presence of HPV DNA was determined by in situ hybridization with biotin-labelled HPV DNA probes. Seventeen tissue specimens were obtained from 9 patients, and included pre-malignant lesions and primary and recurrent tumors. One pre-malignant lesion was positive for papillomavirus structural antigen. This lesion and lesions from 2 other patients hybridized at low stringency (Tm-35 degrees) to 3 different HPV probes. By hybridization under high stringency conditions (Tm-20 degrees), the virus in each case was identified as being HPV2.

摘要

对口腔疣状癌患者的组织进行了人乳头瘤病毒（HPV）检测。通过免疫组织化学染色检测组织中常见乳头瘤病毒抗原类型的存在情况，并通过与生物素标记的HPV DNA探针进行原位杂交来确定HPV DNA的存在。从9名患者身上获取了17个组织标本，包括癌前病变、原发性肿瘤和复发性肿瘤。一个癌前病变的乳头瘤病毒结构抗原呈阳性。该病变以及另外2名患者的病变在低严谨度（Tm - 35摄氏度）下与3种不同的HPV探针杂交。在高严谨度条件（Tm - 20摄氏度）下进行杂交时，每种情况下的病毒均被鉴定为HPV2。