Giraudo A T, Sagedahl A, Bergmann I E, La Torre J L, Scodeller E A
J Virol. 1987 Feb;61(2):419-25. doi: 10.1128/JVI.61.2.419-425.1987.
A guanidine-resistant mutant of the attenuated strain of aphthovirus type 01 strain Campos and the original wild-type strain were crossed to generate recombinant viruses. Two independently derived recombinant viruses were isolated. One isolate (RI) contained the P1 (structural proteins) gene region of attenuated strain and P3 (polymerase precursor) gene region of the wild-type strain. The other isolate (RII) had a genomic structure complementary to that of RI, this is, P1 of the wild-type strain and P3 of the attenuated virus. Recombinant RII inherited some in vitro phenotypic markers that were characteristic of the attenuated strain, whereas the RI recombinant had in vitro behavior that was similar to that of the wild-type strain. The data obtained suggest that the polymerase precursor (P3) of the attenuated strain (01 Campos) could be involved in the determination of the attenuated phenotype for fetal bovine kidney cells and, eventually, for cattle.
口蹄疫病毒01型坎波斯毒株的弱毒株的一种胍抗性突变体与原始野生型毒株杂交，以产生重组病毒。分离出两种独立衍生的重组病毒。一种分离株（RI）包含弱毒株的P1（结构蛋白）基因区域和野生型毒株的P3（聚合酶前体）基因区域。另一种分离株（RII）的基因组结构与RI互补，即野生型毒株的P1和弱毒病毒的P3。重组RII继承了一些弱毒株特有的体外表型标记，而RI重组体的体外行为与野生型毒株相似。所获得的数据表明，弱毒株（01坎波斯）的聚合酶前体（P3）可能参与了胎牛肾细胞以及最终牛的弱毒表型的确定。
