Biomaterial Research Laboratory, Department of Material Science and Engineering, College of Chemistry and Materials, Jinan University, Guangzhou 510632, PR China.
Biomaterial Research Laboratory, Department of Material Science and Engineering, College of Chemistry and Materials, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, PR China.
Mater Sci Eng C Mater Biol Appl. 2018 Dec 1;93:319-331. doi: 10.1016/j.msec.2018.08.006. Epub 2018 Aug 4.
In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too. Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively. Antibacterial experiment against E. coli and S. aureus indicated that antibacterial activity of the G-C-D-PU fibrous membrane was markedly superior to that of pure PU and C-D-PU fibrous membranes. In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L. Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
在这项研究中，为了提高静电纺丝聚氨酯（PU）纤维膜的抗菌活性和细胞相容性，通过聚多巴胺（PDOPA）中间层将季铵化壳寡糖（G-COS）固定在纤维膜表面，得到 G-COS 功能化的 PU（G-C-D-PU）。作为对照，也制备了壳寡糖（COS）功能化的 PU 纤维膜（C-D-PU）。对原始和改性的 PU 纤维膜的表面组成、形貌、亲水性和表面能进行了表征，结果表明，COS 和 G-COS 的改性分别明显增加了 PU 纤维膜的表面粗糙度和亲水性。对大肠杆菌和金黄色葡萄球菌的抗菌实验表明，G-C-D-PU 纤维膜的抗菌活性明显优于纯 PU 和 C-D-PU 纤维膜。体外细胞培养实验表明，通过 PDOPA 和 COS 以及浓度分别为 2 g/L 和 6 g/L 的 G-COS 依次固定，NIH-3T3 细胞在 PU 纤维膜上的黏附和增殖得到改善。此外，G-COS 含量相对较高的 G-C-D-PU 纤维膜更有利于增强抗菌活性，但相反，G-COS 含量相对较低的纤维膜更有利于细胞附着和增殖。
