Kim Jeong In, Pant Hem Raj, Sim Hyun-Jaung, Lee Kang Min, Kim Cheol Sang
Department of Bio-nano System Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Department of Bio-nano System Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Department of Engineering Science and Humanities, Pulchowk Campus, Tribhuvan University, Kathmandu, Nepal; Research Institute for Next Generation, Kalanki, Kathmandu, Nepal.
Mater Sci Eng C Mater Biol Appl. 2014 Nov;44:52-7. doi: 10.1016/j.msec.2014.07.062. Epub 2014 Aug 2.
Tissue engineering requires functional polymeric membrane for adequate space for cell migration and attachment within the nanostructure. Therefore, biocompatible propolis loaded polyurethane (propolis/PU) nanofibers were successfully prepared using electrospinning of propolis/PU blend solution. Here, composite nanofibers were subjected to detailed analysis using electron microscopy, FT-IR spectroscopy, thermal gravimetric analysis (TGA), and mechanical properties and water contact angle measurement. FE-SEM images revealed that the composite nanofibers became point-bonded with increasing amounts of propolis in the blend due to its adhesive properties. Incorporation of small amount of propolis through PU matrix could improve the hydrophilicity and mechanical strength of the fibrous membrane. In order to assay the cytocompatibility and cell behavior on the composite scaffolds, fibroblast cells were seeded on the matrix. Results suggest that the incorporation of propolis into PU fibers could increase its cell compatibility. Moreover, composite nanofibers have effective antibacterial activity. Therefore, as-synthesized nanocomposite fibrous mat has great potentiality in wound dressing and skin tissue engineering.
组织工程需要功能性聚合物膜，以便在纳米结构内为细胞迁移和附着提供足够的空间。因此，通过静电纺丝蜂胶/聚氨酯（蜂胶/PU）共混溶液，成功制备了具有生物相容性的负载蜂胶的聚氨酯（蜂胶/PU）纳米纤维。在此，使用电子显微镜、傅里叶变换红外光谱（FT-IR）、热重分析（TGA）以及机械性能和水接触角测量对复合纳米纤维进行了详细分析。场发射扫描电子显微镜（FE-SEM）图像显示，由于蜂胶的粘附特性，随着共混物中蜂胶含量的增加，复合纳米纤维形成了点粘结。通过聚氨酯基质掺入少量蜂胶可以提高纤维膜的亲水性和机械强度。为了测定复合支架的细胞相容性和细胞行为，将成纤维细胞接种在基质上。结果表明，将蜂胶掺入聚氨酯纤维中可以提高其细胞相容性。此外，复合纳米纤维具有有效的抗菌活性。因此，合成的纳米复合纤维垫在伤口敷料和皮肤组织工程中具有巨大的潜力。
