Liu Hua, Li Wenling, Luo Binghong, Chen Xuexing, Wen Wei, Zhou Changren
Biomaterial Research Laboratory, Department of Material Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, PR China.
Biomaterial Research Laboratory, Department of Material Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:399-409. doi: 10.1016/j.msec.2017.05.077. Epub 2017 May 14.
In this study, icariin (ICA), one of the main active ingredients of Herba Epimedii for osteogenesis, was applied to functionalize electrospinning poly(l-lactide) (PLLA) fibrous membrane via an intermediate layer of polydopamine (PDA) to obtain enhanced cytocompatibility and osteogenic activity. For this purpose, an array of PDA-coated PLLA fibrous membranes (PLLA-0.5PDA, PLLA-1PDA, PLLA-2PDA, PLLA-5PDA) and ICA-modified PLLA-2PDA fibrous membranes (PLLA-2PDA-10ICA, PLLA-2PDA-20ICA, PLLA-2PDA-40ICA) were successively prepared. Successful modification of PDA and ICA onto PLLA fibrous membranes was verified by field emission scanning electron microscope (FESEM), thermogravimetric analysis (TGA) and X-ray photoelectron spectroscopy (XPS). Besides, the hydrophilicity as well as tensile properties of PLLA fibrous membrane were improved after surface modified with PDA and ICA. In vitro cells culture experiments revealed that the adhesion, proliferation and osteogenic differentiation of MC3T3-E1 cells on the PLLA fibrous membrane were significantly improved by successively immobilized with PDA and ICA. Moreover, the concentration of ICA immobilized on the fibrous membranes has the complicated effects on the MC3T3-E1 cells behavior. The PLLA-2PDA-ICA fibrous membranes with low ICA concentration promoted the cell adhesion and proliferation, but on the contrary, those with high ICA concentration were more beneficial to the enhancement in ALP activity and calcium deposition.
在本研究中，淫羊藿促进成骨的主要活性成分之一淫羊藿苷（ICA）通过聚多巴胺（PDA）中间层用于功能化静电纺丝聚左旋乳酸（PLLA）纤维膜，以获得增强的细胞相容性和成骨活性。为此，依次制备了一系列PDA包覆的PLLA纤维膜（PLLA - 0.5PDA、PLLA - 1PDA、PLLA - 2PDA、PLLA - 5PDA）和ICA修饰的PLLA - 2PDA纤维膜（PLLA - 2PDA - 10ICA、PLLA - 2PDA - 20ICA、PLLA - 2PDA - 40ICA）。通过场发射扫描电子显微镜（FESEM）、热重分析（TGA）和X射线光电子能谱（XPS）验证了PDA和ICA成功修饰到PLLA纤维膜上。此外，用PDA和ICA进行表面改性后，PLLA纤维膜的亲水性以及拉伸性能得到改善。体外细胞培养实验表明，依次固定PDA和ICA后，MC3T3 - E1细胞在PLLA纤维膜上的黏附、增殖和成骨分化显著改善。此外，固定在纤维膜上的ICA浓度对MC3T3 - E1细胞行为有复杂影响。低ICA浓度的PLLA - 2PDA - ICA纤维膜促进细胞黏附和增殖，但相反，高ICA浓度的纤维膜更有利于碱性磷酸酶（ALP）活性增强和钙沉积。
