过表达猿猴病毒40大T抗原的小鼠细胞中不允许性的分析。
Analysis of nonpermissivity in mouse cells overexpressing simian virus 40 T antigen.
作者信息
Gerard R D, Guggenheimer R A, Gluzman Y
出版信息
J Virol. 1987 Mar;61(3):851-7. doi: 10.1128/JVI.61.3.851-857.1987.
Abstract
To analyze the nature of the nonpermissivity of mouse cells for simian virus 40 (SV40) DNA replication, we isolated mouse cells producing SV40 T antigen (Tag) at levels equal to or greater than that found in COS1 cells. These mouse cells were nonpermissive for the replication of exogenously added SV40 DNA, although purified Tag isolated from these cells was able to support SV40 DNA replication in vitro. Furthermore, when mouse cells expressing Tag were fused to monkey cells, SV40 DNA replication was observed. These results indicate that the mere production of large quantities of wild-type SV40 Tag does not overcome the block of nonpermissivity in mouse cells and that cellular factors must play a critical role.
摘要
为了分析小鼠细胞对猴病毒40（SV40）DNA复制不许可的本质，我们分离出了产生SV40 T抗原（Tag）水平等于或高于COS1细胞中发现水平的小鼠细胞。这些小鼠细胞对外源添加的SV40 DNA的复制不许可，尽管从这些细胞中分离出的纯化Tag能够在体外支持SV40 DNA复制。此外，当表达Tag的小鼠细胞与猴细胞融合时，观察到了SV40 DNA复制。这些结果表明，仅仅产生大量野生型SV40 Tag并不能克服小鼠细胞中的不许可障碍，并且细胞因子必定起着关键作用。
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