Lafont Maxime, Goncalves Priscila, Guo Ximing, Montagnani Caroline, Raftos David, Green Timothy
Sydney Institute of Marine Science, Chowder Bay, Sydney, Australia; IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, France.
Sydney Institute of Marine Science, Chowder Bay, Sydney, Australia; Macquarie University, Department of Biological Sciences, Sydney, Australia.
Dev Comp Immunol. 2019 Feb;91:17-25. doi: 10.1016/j.dci.2018.09.022. Epub 2018 Sep 29.
The oyster's immune system is capable of adapting upon exposure to a pathogen-associated molecular pattern (PAMP) to have an enhanced secondary response against the same type of pathogen. This has been demonstrated using poly(I:C) to elicit an antiviral response in the Pacific oyster (Crassostrea gigas) against Ostreid herpesvirus (OsHV-1). Improved survival following exposure to poly(I:C) has been found in later life stages (within-generational immune priming) and in the next generation (transgenerational immune priming). The mechanism that the oyster uses to transfer immunity to the next generation is unknown. Here we show that oyster larvae have higher survival to OsHV-1 when their mothers, but not their fathers, are exposed to poly(I:C) prior to spawning. RNA-seq provided no evidence to suggest that parental exposure to poly(I:C) reconfigures antiviral gene expression in unchallenged larvae. We conclude that the improved survival of larvae might occur via maternal provisioning of antiviral compounds in the eggs.
牡蛎的免疫系统能够在接触病原体相关分子模式（PAMP）后进行适应性调整，从而对同类型病原体产生增强的二次反应。这一点已通过使用聚肌胞苷酸（poly(I:C)）在太平洋牡蛎（太平洋牡蛎）中引发抗病毒反应以对抗牡蛎疱疹病毒（OsHV-1）得到证明。在后期生命阶段（代内免疫致敏）和下一代（跨代免疫致敏）中，发现接触聚肌胞苷酸（poly(I:C)）后存活率有所提高。牡蛎将免疫力传递给下一代的机制尚不清楚。在这里，我们表明，当牡蛎幼虫的母亲而非父亲在产卵前接触聚肌胞苷酸（poly(I:C)）时，幼虫对OsHV-1具有更高的存活率。RNA测序没有提供证据表明亲代接触聚肌胞苷酸（poly(I:C)）会重新配置未受挑战幼虫中的抗病毒基因表达。我们得出结论，幼虫存活率的提高可能是通过母体在卵中提供抗病毒化合物实现的。
