Department of Pediatrics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
Department of Pediatrics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
J Photochem Photobiol B. 2018 Dec;189:29-35. doi: 10.1016/j.jphotobiol.2018.07.026. Epub 2018 Jul 27.
We investigated the in vitro mitochondria-mediated anticancer and antiproliferative effects of extracts of Annona glabra leafs on human leukemia cells. A. glabra is a tropical tree that exhibits several clinical and pharmacological properties in humans and is effective against cancer. We investigated the antiproliferative effects of an alcoholic extract of A. glabra on MC-1010 human monocytic leukemia cells (crl-12253) based on phytochemical analyses, cell viability, free radical scavenging activity, intracellular reactive oxygen species (ROS) levels, ATP content, mitochondrial fragmentation, and cell migration assays. The results indicated the presence of flavonoids, terpenoids, glycosides, steroids, saponins, tannins, anthraquinones, and acidic compounds in extracts. Leukemia cell viability was reduced up to 28% after incubation with the extract, while the free radical reducing power and scavenging activity were significantly increased. Higher concentrations of extract significantly inhibited leukemia cell colony formation. ROS increased up to 66% following incubation with extract, while the ATP content decreased up to 31%. Condensed, fragmented, and clumped mitochondria were observed in treated cells. In flow cytometric analyses, 10.7% and 22.4% of cells were apoptotic following incubation with 80 and 100 μg/mL extract, respectively. Moreover, treated leukemia cells exhibited reduced migratory potential. Overall, the results suggest that leaf extracts of A. glabra may act as potential anticancer agents against human leukemia cells.
我们研究了番石榴叶提取物在体外通过线粒体介导的对人类白血病细胞的抗癌和抗增殖作用。番石榴是一种热带树木，在人类中具有多种临床和药理学特性，并且对癌症有效。我们基于植物化学分析、细胞活力、自由基清除活性、细胞内活性氧 (ROS) 水平、ATP 含量、线粒体碎片化和细胞迁移测定，研究了番石榴叶的醇提物对 MC-1010 人类单核细胞白血病细胞 (crl-12253) 的抗增殖作用。结果表明提取物中存在类黄酮、萜类、糖苷、甾体、皂苷、单宁、蒽醌和酸性化合物。用提取物孵育后，白血病细胞活力降低了高达 28%，而自由基还原能力和清除活性显著增加。较高浓度的提取物显著抑制白血病细胞集落形成。孵育后 ROS 增加高达 66%，而 ATP 含量降低高达 31%。在处理的细胞中观察到浓缩、碎片化和聚集的线粒体。在流式细胞术分析中，分别用 80 和 100μg/mL 提取物孵育后，有 10.7%和 22.4%的细胞发生凋亡。此外，经处理的白血病细胞迁移能力降低。总体而言，这些结果表明番石榴叶提取物可能作为潜在的抗癌剂对人类白血病细胞发挥作用。
