Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak 124001, Haryana, India.
Optimization and Mechatronics Laboratory, Department of Mechanical Engineering, University Institute of Engineering and Technology, Maharshi Dayanand University, Rohtak, Haryana, India.
Bioresour Technol. 2019 Jan;271:274-282. doi: 10.1016/j.biortech.2018.09.115. Epub 2018 Sep 22.
The process parameters for xylanase biobleaching of mixed hardwood pulp like, reaction time (6-35 h), pulp consistency (2.5-15%) and xylanase dose (5-35 U) were optimized using OFAT approach and hybrid statistical tools viz. GA-ANN and GA-ANFIS. The biobleaching ability of xylanase in terms of reducing sugar yield increased up to 28.16 mg g (28.05%) than OFAT optimization (21.99 mg g of pulp) after employing hybrid statistical tools. After TCF bleaching of xylanase treated pulp, we observed that lignin content reduced to 0.29% whereas it was still 0.41% in the untreated pulp. Moreover, the brightness level achieved up to 70.4% in xylanase treated pulp while it was only 53.60% in the chemically treated pulp. The kappa number for xylanase treated, chemically treated, and xylanase-chemical treated pulp was recorded 9.90, 7.10 and 4.70, respectively. The present study reports an improved eco-friendly biobleaching method using novel GA-ANN and GA-ANFIS hybrid statistical tools.
使用 OFAT 方法和混合统计工具(GA-ANN 和 GA-ANFIS)对类似混合硬木浆的木聚糖酶生物漂白的过程参数(反应时间(6-35 小时)、纸浆浓度(2.5-15%)和木聚糖酶剂量(5-35U)进行了优化。与 OFAT 优化(21.99mg/g 纸浆)相比,在使用混合统计工具后,木聚糖酶的生物漂白能力在还原糖产率方面提高了 28.16mg/g(28.05%)。在木聚糖酶处理的纸浆进行 TCF 漂白后,我们观察到木质素含量降低到 0.29%,而未处理的纸浆中木质素含量仍为 0.41%。此外,木聚糖酶处理的纸浆的亮度水平达到 70.4%,而化学处理的纸浆仅为 53.60%。木聚糖酶处理、化学处理和木聚糖酶-化学处理的纸浆的卡伯值分别为 9.90、7.10 和 4.70。本研究报告了一种使用新型 GA-ANN 和 GA-ANFIS 混合统计工具改进的环保生物漂白方法。