Shukla Pratyoosh
Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana 124001 India.
Indian J Microbiol. 2019 Dec;59(4):401-409. doi: 10.1007/s12088-019-00819-9. Epub 2019 Aug 23.
Microbial enzymes are high in demand and there is focus on their efficient, cost effective and eco-friendly production. The relevant microbial enzymes for respective industries needs to be identified but the conventional technologies don't have much edge over it. So, there is more attention towards high throughput methods for production of efficient enzymes. The enzymes produced by microbes need to be modified to bear the extreme conditions of the industries in order to get prolific outcomes and here the synthetic biology tools may be augmented to modify such microbes and enzymes. These tools are applied to synthesize novel and efficient enzymes. Use of computational tools for enzyme modification has provided new avenues for faster and specific modification of enzymes in a shorter time period. This review focuses on few important enzymes and their modification through synthetic biology tools including genetic modification, nanotechnology, post translational modification.
微生物酶的需求量很大，人们关注其高效、经济且环保的生产。需要确定各行业相关的微生物酶，但传统技术在这方面优势不大。因此，人们更加关注用于高效酶生产的高通量方法。微生物产生的酶需要经过修饰以适应各行业的极端条件，从而获得丰硕成果，在此过程中可借助合成生物学工具来修饰此类微生物和酶。这些工具用于合成新型高效酶。利用计算工具进行酶修饰为在更短时间内对酶进行快速且特异性的修饰提供了新途径。本综述重点关注几种重要的酶以及通过合成生物学工具对其进行的修饰，包括基因修饰、纳米技术、翻译后修饰。
