Cui Xiangrong, Yi Qin, Jing Xuan, Huang Yue, Tian Jie, Long Chunlan, Xiang Zhongping, Liu Jianping, Zhang Chunmin, Tan Bin, Li Yasha, Zhu Jing
Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China.
China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing, China.
Cell Physiol Biochem. 2018;50(1):41-51. doi: 10.1159/000493956. Epub 2018 Oct 2.
BACKGROUND/AIMS: Maternally expressed gene 3 (MEG3) is an imprinted gene with maternal expression, which may function as a tumor suppressor by inhibiting angiogenesis. To identify the prognostic value of MEG3 in breast cancer, systematic analysis was performed in this study.
To evaluate gene alteration during breast carcinogenesis, we explored MEG3 expression using the Serial Analysis of Gene Expression Genie suite and Oncomine analysis. The prognostic roles of MEG3 in breast cancer were investigated using the PrognoScan database. The heat map and methylation status of MEG3 were determined using the UCSC Genome Browser.
We found that MEG3 was more frequently downregulated in breast cancer than in normal tissues and this correlated with prognosis. However, estrogen receptor and progesterone receptor status were found to be positively correlated with MEG3 expression. Conversely, basal-like status, triple-negative breast cancer status, and Scarff Bloom & Richardson grade criterion were negatively correlated with MEG3 expression. Following data mining in multiple big data databases, we confirmed a positive correlation between MEG3 and heparan sulfate proteoglycan 2 (HSPG2) expression in breast cancer tissues.
MEG3 could be adopted as a marker to predict the prognosis of breast cancer with HSPG2. However, large-scale and comprehensive research is needed to clarify our results.
背景/目的：母源表达基因3（MEG3）是一种具有母源表达的印记基因，可能通过抑制血管生成发挥肿瘤抑制作用。为了确定MEG3在乳腺癌中的预后价值，本研究进行了系统分析。
为了评估乳腺癌发生过程中的基因改变，我们使用基因表达序列分析Genie套件和Oncomine分析来探究MEG3的表达。使用PrognoScan数据库研究MEG3在乳腺癌中的预后作用。使用UCSC基因组浏览器确定MEG3的热图和甲基化状态。
我们发现MEG3在乳腺癌中比在正常组织中更频繁地下调，这与预后相关。然而，发现雌激素受体和孕激素受体状态与MEG3表达呈正相关。相反，基底样状态、三阴性乳腺癌状态和斯卡夫-布卢姆-理查森分级标准与MEG3表达呈负相关。在多个大数据数据库中进行数据挖掘后，我们证实了MEG3与乳腺癌组织中硫酸乙酰肝素蛋白聚糖2（HSPG2）表达之间存在正相关。
MEG3可作为预测乳腺癌与HSPG2预后的标志物。然而，需要大规模和全面的研究来阐明我们的结果。
