在GAL-10启动子控制下酵母中乙肝表面抗原（HBsAg）基因的表达。
The expression of HBsAg gene in yeast under GAL-10 promoter control.
作者信息
Shen L P, Wang E L, Pan T C, Dai P H, Li Z P, Li Y Y
出版信息
Sci Sin B. 1986 Aug;29(8):856-63.
PMID:3027890
Abstract
The surface antigen gene of HBV (adr subtype) is inserted into the YEP 1 which is replicated and selected in E. coli and baker yeast Saccharomyces cerevisiae. The HBsAg gene can be expressed in yeast under GAL-10 promoter control. The protein synthesized in yeast is assembled into particles that have the same size and shape as particles isolated from plasma. One microgram surface antigen can be yielded in 100 ml culture.
摘要
乙型肝炎病毒（adr亚型）的表面抗原基因被插入到YEP1中，该载体在大肠杆菌和酿酒酵母中进行复制和筛选。乙肝表面抗原基因可在GAL-10启动子的控制下在酵母中表达。酵母中合成的蛋白质组装成与从血浆中分离出的颗粒大小和形状相同的颗粒。在100毫升培养物中可产生1微克表面抗原。
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