Miyanohara A, Toh-e A, Nozaki C, Hamada F, Ohtomo N, Matsubara K
Proc Natl Acad Sci U S A. 1983 Jan;80(1):1-5. doi: 10.1073/pnas.80.1.1.
The DNA sequence coding for hepatitis B virus surface antigen (HBsAg) was placed under control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of autonomous replication in both yeast and Escherichia coli. Yeast transformed by this plasmid synthesized up to 5 X 10(5) molecules per cell of immunologically active HBsAg polypeptide in phosphate-free medium. The HBsAg polypeptides produced in the yeast cells were assembled into 20- to 22-nm spherical or oval particles and were immunogenic.
编码乙型肝炎病毒表面抗原（HBsAg）的DNA序列，被置于酿酒酵母可阻遏酸性磷酸酶启动子的控制之下，该启动子位于一个在酵母和大肠杆菌中均能自主复制的质粒中。用这种质粒转化的酵母，在无磷酸盐培养基中，每个细胞可合成多达5×10⁵个免疫活性HBsAg多肽分子。酵母细胞中产生的HBsAg多肽组装成20至22纳米的球形或椭圆形颗粒，且具有免疫原性。
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