乙型肝炎病毒表面抗原颗粒在酵母中的合成与组装。
Synthesis and assembly of hepatitis B virus surface antigen particles in yeast.
作者信息
Valenzuela P, Medina A, Rutter W J, Ammerer G, Hall B D
出版信息
Nature. 1982 Jul 22;298(5872):347-50. doi: 10.1038/298347a0.
DOI:10.1038/298347a0
PMID:7045698
Abstract
The surface antigens of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5'-flanking region of yeast alcohol dehydrogenase I as a promotor to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.
摘要
通过使用一种表达载体，利用酵母乙醇脱氢酶I的5'侧翼区域作为启动子来转录表面抗原编码序列，已在酿酒酵母中合成了乙型肝炎病毒的表面抗原（HBsAg）。在酵母中合成的蛋白质组装成具有与人细胞分泌的22纳米颗粒相似特性的颗粒。
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