School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.
School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.
Bioresour Technol. 2018 Dec;270:755-761. doi: 10.1016/j.biortech.2018.09.101. Epub 2018 Sep 21.
Freshwater-derived anaerobic ammonia oxidation (anammox) bacteria ("Candidatus Brocadia sinica") were investigated to remove nitrogen from high-salinity and low-temperature wastewater with glycine addition. The reactor was operated at 15 ± 0.5 °C with influent pH of 7.5 ± 0.1. When glycine were 0.2, 0.4, and 0.6 mM, respectively, nitrite removal rate (NRR) increased by 27.7%, 47.3%, and 70.4% accordingly. Optimal ammonia removal rate (0.32 kg/(m·d)) and NRR (0.45 kg/(m·d)) were achieved at 0.8 mM glycine. Effect resulting from glycine on nitrite reductase was higher than hydrazine synthase. Moreover, ΔNO-N/ΔNH-N increased with glycine addition while ΔNO-N/ΔNH-N first increased and then decreased. The remodified Logistic model and modified Boltzmann model were appropriate to describe nitrogen removal with glycine addition. Kinetic parameter λ achieved through the remodified Logistic model revealed that "Candidatus Brocadia sinica" had a shorter lag phase than that of marine anammox bacteria.
采用添加甘氨酸的方法，利用淡水源厌氧氨氧化（anammox）细菌（“Candidatus Brocadia sinica”）从高盐低温废水中去除氮。反应器在 15 ± 0.5°C 下运行，进水 pH 值为 7.5 ± 0.1。当甘氨酸分别为 0.2、0.4 和 0.6 mM 时，亚硝酸盐去除率（NRR）相应增加了 27.7%、47.3%和 70.4%。在 0.8 mM 甘氨酸时，氨去除率（0.32 kg/（m·d））和 NRR（0.45 kg/（m·d））达到最佳。甘氨酸对亚硝酸盐还原酶的影响高于肼合酶。此外，随着甘氨酸的添加，ΔNO-N/ΔNH-N 增加，而 ΔNO-N/ΔNH-N 先增加后减少。添加甘氨酸的氮去除的修正 Logistic 模型和修正 Boltzmann 模型较为合适。通过修正的 Logistic 模型得到的动力学参数 λ 表明，“Candidatus Brocadia sinica”的迟滞期比海洋 anammox 细菌短。
