Wangrawa Dimitri Wendgida, Badolo Athanase, Ilboudo Zakaria, Guelbéogo Wamdaogo Moussa, Kiendrébeogo Martin, Nébié Roger Charles Honorat, Sagnon N'Falé, Sanon Antoine
Laboratoire d'Entomologie Fondamentale et Appliquée, Université Ouaga I Pr Joseph, Ouagadougou, Burkina Faso.
Unité de Formation et de Recherches/Sciences et Technologies, Université Norbert ZONGO, Koudougou, Burkina Faso.
J Econ Entomol. 2018 Dec 14;111(6):2844-2853. doi: 10.1093/jee/toy276.
The emergence and intensification of resistance to insecticides in malaria vector populations is the main obstacle to insecticide-based control efforts. The main objective of this study was to evaluate the larvicidal and adulticidal properties of the essential oils (EOs) of Ocimum canum, Hyptis suaveolens, Hyptis spicigera, and Lantana camara on field-collected, pyrethroids-resistant mosquitoes, local laboratory strains, and susceptible 'Kisumu' strain of Anopheles gambiae (Meigen) (Diptera: Culicidae) populations. Larvae and adults of these mosquitoes were challenged against four EOs. The mortality rates of larvae and adults were assessed 24 h after exposure to the EOs. Species identifications and detection of the L1014F and L1014S kdr mutations and the 1575Y super-kdr mutation were carried out using polymerase chain reaction on the pyrethroid-resistant mosquitoes from the field. EO compositions were analyzed by gas chromatography and mass spectrometry. Monoterpene hydrocarbons were the major components of H. suaveolens and H. spicigera EOs (49.8%) and (69.6%), respectively, whereas oxygenated monoterpenes (68.7%) were predominant in the O. canum EO. For L. camara, the component yields were variable, but it was the most effective EO against all strains. The LC50 values for the larvae were 7.73 and 25.63 ppm for the susceptible 'Kisumu' and resistant field strains, respectively. The LC50 for adults was 0.24% for the susceptible strain and 1.98% for the resistant strain. Molecular analysis confirmed the presence of L1014F and N1575Y mutations in resistant Anopheles arabiensis and Anopheles coluzzii mosquitoes from the field. Our results highlighted the potential of the EOs of local plants as insecticides against resistant and susceptible strains of An. gambiae populations.
疟疾病媒种群对杀虫剂的抗药性出现并加剧，这是基于杀虫剂的防治工作的主要障碍。本研究的主要目的是评估毛罗勒、香彩雀、穗状香彩雀和马缨丹的精油对野外采集的、对拟除虫菊酯具有抗性的蚊子、当地实验室菌株以及冈比亚按蚊（梅根）（双翅目：蚊科）的敏感“基苏木”菌株种群的杀幼虫和杀成虫特性。这些蚊子的幼虫和成虫用四种精油进行处理。暴露于精油24小时后评估幼虫和成虫的死亡率。使用聚合酶链反应对野外采集的对拟除虫菊酯具有抗性的蚊子进行物种鉴定，并检测L1014F和L1014S击倒抗性（kdr）突变以及1575Y超级kdr突变。通过气相色谱和质谱分析精油成分。单萜烃分别是香彩雀和穗状香彩雀精油的主要成分（49.8%）和（69.6%），而含氧单萜（68.7%）在毛罗勒精油中占主导地位。对于马缨丹，成分产量各不相同，但它是对所有菌株最有效的精油。敏感“基苏木”菌株和抗性野外菌株幼虫的半数致死浓度（LC50）值分别为7.73 ppm和25.63 ppm。敏感菌株成虫的LC50为0.24%，抗性菌株为1.98%。分子分析证实野外采集的阿拉伯按蚊和科氏按蚊抗性蚊子中存在L1014F和N1575Y突变。我们的结果突出了当地植物精油作为杀虫剂对抗冈比亚按蚊抗性和敏感菌株种群的潜力。
