Department of Bioengineering, University of California, Los Angeles, Los Angeles, California 90095, USA.
Division of Advanced Prosthodontics and the Weintraub Center for Reconstructive Biotechnology, University of California, Los Angeles, Los Angeles, California 90095, USA.
J Biomed Mater Res B Appl Biomater. 2019 Jul;107(5):1384-1392. doi: 10.1002/jbm.b.34230. Epub 2018 Oct 3.
Indocyanine green (ICG) is the only FDA-approved near-infrared dye and it is currently used clinically for diagnostic applications. However, there is significant interest in using ICG for triggered drug delivery applications and heat ablation therapy. Unfortunately, free ICG has a short half-life in vivo and is rapidly cleared from circulation. Liposomes have been frequently used to improve ICG's stability and overall time of effectiveness in vivo, but they have limited stability due to the susceptibility of phospholipids to hydrolysis and oxidation. In this study, nonphospholipid liposomes were used to encapsulate ICG, and the resulting liposomes were characterized for size, encapsulation efficiency, stability, and photothermal response. Using the thin-film hydration method, an ICG encapsulation efficiency of 54% was achieved, and the liposomes were stable for up to 12 weeks, with detectable levels of encapsulated ICG up to week 4. Additionally, ICG-loaded liposomes were capable of rapidly producing a significant photothermal response upon exposure to near-infrared light, and this photothermal response was able to induce changes in the mechanical properties of thermally responsive hydrogels. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1384-1392, 2019.
吲哚菁绿（ICG）是唯一获得美国食品和药物管理局批准的近红外染料，目前临床上用于诊断应用。然而，人们对将 ICG 用于触发药物输送应用和热消融治疗有着浓厚的兴趣。不幸的是，游离 ICG 在体内半衰期短，会迅速从循环中清除。脂质体经常被用于提高 ICG 在体内的稳定性和整体有效性，但由于磷脂易发生水解和氧化，它们的稳定性有限。在这项研究中，使用非磷脂脂质体来包裹 ICG，并对其大小、包封效率、稳定性和光热响应进行了表征。采用薄膜水化法，实现了 54%的 ICG 包封效率，脂质体在长达 12 周的时间内保持稳定，可检测到包封的 ICG 长达 4 周。此外，负载 ICG 的脂质体在近红外光照射下能够迅速产生显著的光热响应，这种光热响应能够诱导热响应水凝胶的机械性能发生变化。2018 年 Wiley 期刊出版公司。J 生物材料研究杂志 B：应用生物材料 107B：1384-1392，2019 年。
