Linsley Chase S, Quach Viola Y, Agrawal Gaurav, Hartnett Elyse, Wu Benjamin M
Department of Bioengineering, University of California, Los Angeles, 420 Westwood Plaza, Room 5121, Engineering V., P.O. Box: 951600, Los Angeles, CA, 90095-1600, USA.
Division of Advanced Prosthodontics and the Weintraub Center for Reconstructive Biotechnology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Drug Deliv Transl Res. 2015 Dec;5(6):611-24. doi: 10.1007/s13346-015-0260-0.
The need for temporal-spatial control over the release of biologically active molecules has motivated efforts to engineer novel drug delivery-on-demand strategies actuated via light irradiation. Many systems, however, have been limited to in vitro proof-of-concept due to biocompatibility issues with the photo-responsive moieties or the light wavelength, intensity, and duration. To overcome these limitations, this paper describes a light actuated drug delivery-on-demand strategy that uses visible and near-infrared (NIR) light and biocompatible chromophores: cardiogreen, methylene blue, and riboflavin. All three chromophores are capable of significant photothermal reaction upon exposure to NIR and visible light, and the amount of temperature change is dependent upon light intensity, wavelength as well as chromophore concentration. Pulsatile release of bovine serum albumin (BSA) from thermally responsive hydrogels was achieved over 4 days. These findings have the potential to translate light-actuated drug delivery-on-demand systems from the bench to clinical applications that require explicit control over the presentation of biologically active molecules.
对生物活性分子释放进行时空控制的需求推动了人们致力于设计通过光照射驱动的新型按需给药策略。然而，由于光响应部分与光波长、强度和持续时间存在生物相容性问题，许多系统仅限于体外概念验证。为了克服这些限制，本文描述了一种光驱动的按需给药策略，该策略使用可见光和近红外（NIR）光以及生物相容性发色团：心绿、亚甲蓝和核黄素。所有这三种发色团在暴露于近红外光和可见光时都能够发生显著的光热反应，温度变化量取决于光强度、波长以及发色团浓度。在4天内实现了牛血清白蛋白（BSA）从热响应水凝胶中的脉冲式释放。这些发现有可能将光驱动的按需给药系统从实验室转化为需要对生物活性分子的呈现进行明确控制的临床应用。
