Zhang Zheng, Yang Jun, Yang Qing, Tian Guangyong, Cui Zhong-Kai
State Key Laboratory for Biology of Plant Diseases and Insect Pests, School of Bioengineering, Dalian University of Technology, Dalian, China.
Institute of Plant Protection and Shenzhen Agricultural Genome Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Mol Biosci. 2021 Mar 30;8:627817. doi: 10.3389/fmolb.2021.627817. eCollection 2021.
Liposome nanocarriers can be used to solve problems of pesticide instability, rapid degradation and a short period of efficacy. Cymoxanil with antifungal activity requires an ideal drug loading system due to its degradation issues. In this paper, cholesterol and stearylamine were used to prepare non-phospholipid liposomes (sterosomes) as a pesticide nanocarrier, and were characterized with field emission scanning electron microscopy (FE-SEM), X-ray powder diffraction (XRD), Fourier-transform infrared (FT-IR) spectrometer, size distribution, and ζ-potential. The results showed sterosomes were successfully loaded with cymoxanil. The loading efficiency and the drug-to-lipid ratio were 92.6% and 0.0761, respectively. Prolonged drug release was obtained for 3 days, improving the short duration of the drug itself. The addition of cymoxanil-loaded sterosomes in culture medium effectively inhibited the growth of yeast cells, which serve as model fungal targets. Sterosomes as nanocarriers significantly improved the stability and efficacy of cymoxanil, thus introducing practical and economically desirable strategies for the preparation of novel pesticide formulations.
脂质体纳米载体可用于解决农药稳定性差、快速降解和药效期短等问题。具有抗真菌活性的霜脲氰由于其降解问题，需要一种理想的载药系统。本文采用胆固醇和硬脂胺制备非磷脂脂质体（甾体脂质体）作为农药纳米载体，并通过场发射扫描电子显微镜（FE-SEM）、X射线粉末衍射（XRD）、傅里叶变换红外（FT-IR）光谱仪、粒径分布和ζ电位对其进行表征。结果表明，霜脲氰成功负载于甾体脂质体中。负载效率和药物-脂质比分别为92.6%和0.0761。药物释放延长了3天，改善了药物本身的短时效问题。在培养基中添加负载霜脲氰的甾体脂质体可有效抑制作为模型真菌靶点的酵母细胞的生长。甾体脂质体作为纳米载体显著提高了霜脲氰的稳定性和药效，从而为新型农药制剂的制备引入了实用且经济可行的策略。
