Dunlop M B, Doherty P C, Zinkernagel R M, Blanden R V
Immunology. 1977 Sep;33(3):361-8.
Spleen cells from CBA/H mice pre-primed intravenously at various intervals with lymphocytic choriomeningitis (LCM) virus were tested for their capacity to respond and generate cytotoxic effector T cells on secondary stimulation both and . secondary stimulation was performed by culturing preprimed spleen cells with infected, syngeneic, peritoneal stimulator' cells for periods of up to 7 days at 37°. Controls were incubated with uninfected stimulators'. secondary stimulation was obtained by intravenous transfer of pre-primed spleen cells into irradiated, heavily infected CBA/H recipients at various times prior to assay of recipients' spleens. Controls were uninfected, irradiated recipients. Effectors were assayed against LCM virus-infected or uninfected, H-2 compatible L929 target cells in a Cr release assay. Spleen cells gave three different patterns of cytotoxic T cell response following or stimulation, depending upon the interval between priming and secondary stimulation. Populations taken from mice approximately days 2–5 post-infection (PI) developed increasing cytotoxic activity against virus-infected targets when cultured with infected or uninfected peritoneal cells, or when transferred into irradiated, uninfected recipients. However, these early populations did not generate cytotoxic activity when transferred into irradiated, heavily infected recipients. Established primary effector populations (i.e. approx. days 7–11 PI) exhibited continuing effector activity when maintained or , more so when peritoneal `stimulator' cells or irradiated recipients were infected. Memory populations (i.e. more than approx. day 13 PI) developed highly potent secondary effectors when cultured with infected peritoneal cells, or on transfer to irradiated, infected recipients. The three different patterns of cytotoxic T cell response of the different preprimed spleen cell populations can be interpreted as indicating three different phases of functional activity of the same population of antigen-reactive T cells from LCM virus-primed mice.
用淋巴细胞性脉络丛脑膜炎（LCM）病毒在不同间隔时间经静脉预先致敏的CBA/H小鼠的脾细胞，在再次刺激时检测其产生细胞毒性效应T细胞的反应能力。再次刺激是通过将预先致敏的脾细胞与感染的、同基因的腹膜“刺激”细胞在37℃下培养长达7天来进行的。对照组与未感染的“刺激”细胞一起孵育。再次刺激也可通过在检测受体脾脏之前的不同时间，将预先致敏的脾细胞静脉内转移到经照射的、严重感染的CBA/H受体中来实现。对照组是未感染的、经照射的受体。效应细胞在铬释放试验中针对LCM病毒感染或未感染的、H-2相容的L929靶细胞进行检测。根据初次致敏和再次刺激之间的间隔时间，脾细胞在初次致敏或再次刺激后呈现出三种不同模式的细胞毒性T细胞反应。感染后约2 - 5天（PI）从小鼠获取的细胞群体，当与感染或未感染的腹膜细胞一起培养时，或转移到经照射的、未感染的受体中时，对病毒感染的靶细胞产生的细胞毒性活性不断增加。然而，这些早期细胞群体转移到经照射的、严重感染的受体中时不会产生细胞毒性活性。已建立的主要效应细胞群体（即感染后约7 - 11天PI）在维持初次致敏或再次刺激时表现出持续的效应活性，当腹膜“刺激”细胞或经照射的受体被感染时活性更强。记忆细胞群体（即感染后超过约13天PI）在与感染的腹膜细胞一起培养时，或转移到经照射的、感染的受体中时，会产生高效的二次效应细胞。不同预先致敏的脾细胞群体的三种不同模式的细胞毒性T细胞反应可解释为表明来自LCM病毒致敏小鼠的同一群抗原反应性T细胞功能活性的三个不同阶段。
