Volkert M, Marker O, Bro-Jorgensen K
J Exp Med. 1974 May 1;139(5):1329-43. doi: 10.1084/jem.139.5.1329.
In this report seven different parameters were employed to investigate the spleen and lymph node cells from mice at the early and the late state of immunity to the lymphocytic choriomeningitis (LCM) virus. Distinct differences were observed. Morphological studies revealed a different size distribution of the cells in the preparations from the early and the late state of immunity. The cell mixtures of early immune cells contained many more large and blast-like lymphoid cells than the other. Where the cell function was concerned, the cytotoxic activity against LCM virus-infected target cells was almost entirely a function of the early immune cells, and our data strongly indicate that enhancement does not play any role for the disappearance by time of this cell activity. The antiviral effect after transfer to acutely infected animals was also predominantly a function of the early immune cells and the same was the case concerning the ability to protect against a lethal acute infection. However, the early immune cells were almost inactive after transfer to chronically infected virus carriers, whereas transplants of late immune cells to such mice had a very strong antiviral effect. The resistance to X irradiation also varied. Even high X-ray doses could not destroy the function of early immune cells, whereas the function of the late immune cells was readily impaired by X-ray treatment. The early and the late immune cells have one thing in common-both are susceptible to treatment with anti-theta serum. Because of the differences observed between the early and the late immune cells, it is concluded that they belong to different cell populations. However, because of the common susceptibility to anti-theta serum, probably both populations are T-cell lymphocytes. The implications of the results and the role of the different cells in the combat of the viral infection are discussed.
在本报告中，采用了七个不同参数来研究淋巴细胞性脉络丛脑膜炎（LCM）病毒免疫早期和晚期小鼠的脾脏和淋巴结细胞。观察到了明显差异。形态学研究显示，免疫早期和晚期制备物中的细胞大小分布不同。早期免疫细胞的细胞混合物中含有比另一种更多的大的和母细胞样淋巴细胞。就细胞功能而言，对LCM病毒感染靶细胞的细胞毒性活性几乎完全是早期免疫细胞的功能，并且我们的数据强烈表明增强作用对这种细胞活性随时间消失不起任何作用。转移到急性感染动物后，抗病毒作用也主要是早期免疫细胞的功能，对致死性急性感染的保护能力也是如此。然而，早期免疫细胞转移到慢性感染病毒携带者后几乎无活性，而将晚期免疫细胞移植到此类小鼠中则具有非常强的抗病毒作用。对X射线的抗性也有所不同。即使高剂量X射线也不能破坏早期免疫细胞的功能，而晚期免疫细胞的功能很容易因X射线处理而受损。早期和晚期免疫细胞有一个共同点——两者都易受抗θ血清处理的影响。由于早期和晚期免疫细胞之间观察到的差异，得出结论它们属于不同的细胞群体。然而，由于对抗θ血清的共同易感性，可能这两个群体都是T细胞淋巴细胞。讨论了结果的意义以及不同细胞在对抗病毒感染中的作用。
