Allison Abimbola, Chowdhury Shahid, Fouladkhah Aliyar
Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA.
Cooperative Extension Program, Tennessee State University, Nashville, TN 37209, USA.
Microorganisms. 2018 Oct 3;6(4):102. doi: 10.3390/microorganisms6040102.
As many as 99% of illnesses caused by are foodborne in nature, leading to 94% hospitalizations, and are responsible for the collective annual deaths of 266 American adults. The current study is a summary of microbiological hurdle validation studies to investigate synergism of mild heat (up to 55 °C) and elevated hydrostatic pressure (up to 380 MPa) for decontamination of and natural background microflora in raw milk and phosphate-buffered saline. At 380 MPa, for treatments of 0 to 12 min, -values of 3.47, 3.15, and 2.94 were observed for inactivation of the pathogen at 4, 25, and 50 °C. Up to 3.73 and >4.26 log CFU/mL reductions ( 0.05) of habituated were achieved using pressure at 380 MPa for 3 and 12 min, respectively. Similarly, background microflora counts were reduced ( 0.05) by 1.3 and >2.4 log CFU/mL after treatments at 380 MPa for 3 and 12 min, respectively. Treatments below three min were less efficacious ( ≥ 0.05) against the pathogen and background microflora, in the vast majority of time and pressure combinations. Results of this study could be incorporated as part of a risk-based food safety management system and risk assessment analyses for mitigating the public health burden of listeriosis.
高达99%由[病原体名称未给出]引起的疾病本质上是食源性的，导致94%的住院治疗，并造成每年266名美国成年人的死亡。本研究是微生物障碍验证研究的总结，旨在调查温和加热（最高55°C）和升高的静水压力（最高380 MPa）对生牛奶和磷酸盐缓冲盐水中[病原体名称未给出]及天然背景微生物的去污协同作用。在380 MPa下，对于0至12分钟的处理，在4、25和50°C时观察到该病原体失活的-log值分别为3.47、3.15和2.94。使用380 MPa的压力分别处理3分钟和12分钟，可使适应性[病原体名称未给出]分别减少高达3.73和>4.26 log CFU/mL（P<0.05）。同样，在380 MPa下分别处理3分钟和12分钟后，背景微生物数量分别减少了1.3和>2.4 log CFU/mL（P<0.05）。在绝大多数时间和压力组合下，低于3分钟的处理对病原体和背景微生物的效果较差（P≥0.05）。本研究结果可纳入基于风险的食品安全管理系统和风险评估分析中，以减轻李斯特菌病对公共卫生的负担。
