Liao Ai-Ho, Chuang Ho-Chiao, Chang Bo-Ya, Kuo Wen-Chuan, Wang Chih-Hung, Gao Hong-Wei, Chiang Chien-Ping
Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.
Department of Biomedical Engineering, National Defense Medical Center, Taipei 11490, Taiwan.
Pharmaceutics. 2018 Oct 3;10(4):175. doi: 10.3390/pharmaceutics10040175.
The optodynamic process of laser-induced microbubble (MB) cavitation in liquids is utilized in various medical applications. However, how incident laser radiation interacts with MBs as an ultrasound contrast agent is rarely estimated when the liquid already contains stable MBs. The present study investigated the efficacy of the laser-mediated cavitation of albumin-shelled MBs in enhancing transdermal drug delivery. Different types and conditions of laser-mediated inertial cavitation of MBs were first evaluated. A CO₂ fractional pulsed laser was selected for combining with MBs in the in vitro and in vivo experiments. The in vitro skin penetration by β-arbutin after 2 h was 2 times greater in the group combining a laser with MBs than in the control group. In small-animal experiments, the whitening effect on the skin of C57BL/6J mice in the group combining a laser with MBs on the skin plus penetrating β-arbutin increased (significantly) by 48.0% at day 11 and 50.0% at day 14, and then tended to stabilize for the remainder of the 20-day experimental period. The present results indicate that combining a CO₂ laser with albumin-shelled MBs can increase skin permeability so as to enhance the delivery of β-arbutin to inhibit melanogenesis in mice without damaging the skin.
激光诱导液体中微泡（MB）空化的光动力学过程被应用于各种医学领域。然而，当液体中已经含有稳定的微泡时，作为超声造影剂的入射激光辐射与微泡之间的相互作用却很少被评估。本研究探讨了激光介导的白蛋白包壳微泡空化在增强透皮给药方面的效果。首先评估了不同类型和条件下激光介导的微泡惯性空化。在体外和体内实验中选择了二氧化碳分数脉冲激光与微泡相结合。在联合激光与微泡的组中，2小时后β-熊果苷的体外皮肤渗透率是对照组的2倍。在小动物实验中，联合激光与微泡并在皮肤上涂抹β-熊果苷的组中，C57BL/6J小鼠皮肤的美白效果在第11天显著增加了48.0%，在第14天增加了50.0%，然后在20天实验期的剩余时间里趋于稳定。目前的结果表明，将二氧化碳激光与白蛋白包壳微泡相结合可以增加皮肤通透性，从而增强β-熊果苷的递送，以抑制小鼠黑色素生成，同时不损伤皮肤。
