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. 中MYB基因家族及其在非生物胁迫和激素处理下的表达的综合分析

Comprehensive Analysis of MYB Gene Family and Their Expressions Under Abiotic Stresses and Hormone Treatments in .

作者信息

Zhang Tengqian, Zhao Yulin, Wang Yucheng, Liu Zhongyuan, Gao Caiqiu

机构信息

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.

Taiyuan Botanical Garden, Taiyuan, China.

出版信息

Front Plant Sci. 2018 Sep 19;9:1303. doi: 10.3389/fpls.2018.01303. eCollection 2018.

Abstract

The MYB transcription factors (TFs) is a plant TF families, which involves in hormone signal transduction, and abiotic stress tolerance, etc. However, there are few studies on the MYB TFs family and its regulatory mechanism in . In this study, 14 genes (named ) were cloned and characterized from . The transcription profiles of in under different abiotic stress conditions were monitored using qRT-PCR. Most of studied were significantly downregulated and/or upregulated by salt and osmotic stress, ABA, GA3 and JA treatments in at least one organ. Especially, was induced in the leaves and roots of when exposed to NaCl treatment at all study periods, indicating that it may involve in salt stress. To further study 3 function, 3 overexpression and knock-down plants and control plants transformed with an empty pROKII were obtained using a transient transformation system. Overexpression of 3 in displayed the lowest O, HO and MDA accumulation, minimal cell death, the most stable K/Na ratio and the lowest electrolyte leakage rate among the three kinds of transient expression in . Conversely, the RNAi-silencing, transiently transformed plants displayed the opposite physiological changes. Therefore, 3 might play a role in salt stress tolerance in transgenic plants.

摘要

MYB转录因子(TFs)是一个植物TF家族,参与激素信号转导以及非生物胁迫耐受性等过程。然而,关于MYB TFs家族及其在[具体植物名称未给出]中的调控机制的研究较少。在本研究中,从[具体植物名称未给出]中克隆并鉴定了14个基因(命名为[具体基因名称未给出])。使用qRT-PCR监测了[具体植物名称未给出]在不同非生物胁迫条件下[具体基因名称未给出]的转录谱。在至少一个器官中,大多数研究的[具体基因名称未给出]在盐胁迫、渗透胁迫、脱落酸(ABA)、赤霉素(GA3)和茉莉酸(JA)处理下显著下调和/或上调。特别是,在所有研究时期,当[具体植物名称未给出]暴露于NaCl处理时,[具体基因名称未给出]在叶片和根中被诱导,表明它可能参与盐胁迫。为了进一步研究[具体基因名称未给出]的功能,使用瞬时转化系统获得了[具体基因名称未给出]过表达和敲低植株以及用空pROKII转化的对照植株。在[具体植物名称未给出]中过表达[具体基因名称未给出]在三种瞬时表达类型中显示出最低的超氧阴离子(O)、过氧化氢(HO)和丙二醛(MDA)积累,最小的细胞死亡,最稳定的钾/钠比和最低的电解质渗漏率。相反,RNA干扰沉默的瞬时转化植株表现出相反的生理变化。因此,[具体基因名称未给出]可能在转基因[具体植物名称未给出]植株的盐胁迫耐受性中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26c3/6156436/749824dbfba0/fpls-09-01303-g001.jpg

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