Liu Zhongyuan, Xie Qingjun, Tang Feifei, Wu Jing, Dong Wenfang, Wang Chao, Gao Caiqiu
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Front Plant Sci. 2021 Jan 15;11:597480. doi: 10.3389/fpls.2020.597480. eCollection 2020.
The salt overly sensitive (SOS) signal transduction pathway is one of the most highly studied salt tolerance pathways in plants. However, the molecular mechanism of the salt stress response in has remained largely unclear. In this study, five genes (-) from were cloned and characterized. The expression levels of most genes significantly changed after NaCl, PEG, and abscisic acid (ABA) treatment in at least one organ. Notably, the expression of was significantly downregulated after 6 h under salt stress. To further analyze function, overexpression and RNAi-mediated silencing were performed using a transient transformation system. Compared with controls, -overexpressing transgenic plants exhibited greater reactive oxygen species (ROS)-scavenging capability and antioxidant enzyme activity, lower malondialdehyde (MDA) and HO levels, and lower electrolyte leakage rates under salt stress. Similar results were obtained for physiological parameters in transgenic Arabidopsis, including HO and MDA accumulation, superoxide dismutase (SOD) and peroxidase (POD) activity, and electrolyte leakage. In addition, transgenic Arabidopsis plants overexpressing displayed increased root growth and fresh weight gain under salt stress. Together, these data suggest that overexpression of confers salt stress tolerance on plants by enhancing antioxidant enzyme activity, improving ROS-scavenging capability, and decreasing the MDA content and lipid peroxidation of cell membranes. These results suggest that might play an important physiological role in salt tolerance in transgenic plants. This study provides a foundation for further elucidation of salt tolerance mechanisms involving s in .
盐过度敏感（SOS）信号转导途径是植物中研究最为深入的耐盐途径之一。然而，[植物名称]中盐胁迫响应的分子机制在很大程度上仍不清楚。在本研究中，从[植物名称]克隆并鉴定了五个[基因名称]基因（[基因具体名称]）。在至少一个器官中，大多数[基因名称]基因的表达水平在NaCl、聚乙二醇（PEG）和脱落酸（ABA）处理后发生了显著变化。值得注意的是，在盐胁迫6小时后，[基因名称]的表达显著下调。为了进一步分析[基因名称]的功能，使用瞬时转化系统进行了[基因名称]过表达和RNA干扰介导的沉默实验。与对照相比，过表达[基因名称]的转基因[植物名称]植株在盐胁迫下表现出更强的活性氧（ROS）清除能力和抗氧化酶活性，丙二醛（MDA）和H₂O₂水平更低，电解质渗漏率更低。转基因拟南芥的生理参数也得到了类似结果，包括H₂O₂和MDA积累、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性以及电解质渗漏。此外，过表达[基因名称]的转基因拟南芥植株在盐胁迫下根系生长增加，鲜重增加。综上所述，这些数据表明，[基因名称]的过表达通过增强抗氧化酶活性、提高ROS清除能力以及降低MDA含量和细胞膜脂质过氧化作用赋予植物耐盐胁迫能力。这些结果表明，[基因名称]可能在转基因[植物名称]的耐盐性中发挥重要的生理作用。本研究为进一步阐明涉及[植物名称]中[基因名称]的耐盐机制奠定了基础。
