Jamialahmadi Khadijeh, Salari Sofia, Alamolhodaei Nafiseh Sadat, Avan Amir, Gholami Leila, Karimi Gholamreza
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
J Pharmacopuncture. 2018 Sep;21(3):177-184. doi: 10.3831/KPI.2018.21.021. Epub 2018 Sep 30.
Auraptene, a natural citrus coumarin, found in plants of Rutaceae and Apiaceae families. In this study, we investigated the effects of auraptene on tumor migration, invasion and matrix metalloproteinase (MMP)-2 and -9 enzymes activity.
The effects of auraptene on the viability of A2780 and Hela cell lines was evaluated by MTT assay. Wound healing migration assay and Boyden chamber assay were determined the effect of auraptene on migration and cell invasion, respectively. MMP-2 and MMP-9 activities were analyzed by gelatin zymography assay.
Auraptene reduced A2780 cell viability. The results showed that auraptene inhibited in vitro migration and invasion of both cells. Furthermore, cell invasion ability suppressed at 100μM auraptene in Hela cells and at 25, 50μM in A2780 cell line. Gelatin zymography showed that for Hela cell line, auraptene suppressed MMP-2 enzymatic activity in all concentrations and for MMP-9 at a concentration between 12.5 to 100μM in A2780 cell line.
Auraptene inhibited migration and invasion of human cervical and ovarian cancer cells in vitro by possibly inhibitory effects on MMP-2 and MMP-9 activity.
奥罗普特因是一种天然柑橘香豆素，存在于芸香科和伞形科植物中。在本研究中，我们调查了奥罗普特因对肿瘤迁移、侵袭以及基质金属蛋白酶（MMP）-2和-9酶活性的影响。
通过MTT法评估奥罗普特因对A2780和Hela细胞系活力的影响。分别采用伤口愈合迁移试验和博伊登小室试验测定奥罗普特因对迁移和细胞侵袭的影响。通过明胶酶谱法分析MMP-2和MMP-9的活性。
奥罗普特因降低了A2780细胞的活力。结果表明，奥罗普特因抑制了两种细胞的体外迁移和侵袭。此外，在Hela细胞中，100μM的奥罗普特因抑制细胞侵袭能力，在A2780细胞系中，25μM和50μM的奥罗普特因抑制细胞侵袭能力。明胶酶谱显示，对于Hela细胞系，奥罗普特因在所有浓度下均抑制MMP-2酶活性，对于A2780细胞系，在12.5至100μM的浓度下抑制MMP-9酶活性。
奥罗普特因可能通过对MMP-2和MMP-9活性的抑制作用，在体外抑制人宫颈癌和卵巢癌细胞的迁移和侵袭。
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