Taylor C R, Gordon I L, Rembaum A, Russell R, Parker J, O'Brien R L, Lukes R J
J Immunol Methods. 1977;17(1-2):81-9. doi: 10.1016/0022-1759(77)90079-5.
A mixed antiglobulin procedure has been developed to provide simultaneous study of cell surface immunoglobulin and detailed cytological features in Giemsa stained preparations. The percentage of surface immunoglobulin bearing lymphocytes, monocytes and granulocytes changed dramatically when lymphocytes were incubated at 37 degrees C, or following sheep erythrocyte rosette enrichment procedures.
已开发出一种混合抗球蛋白程序,用于在吉姆萨染色制剂中同时研究细胞表面免疫球蛋白和详细的细胞学特征。当淋巴细胞在37℃孵育时,或在绵羊红细胞花环富集程序之后,带有表面免疫球蛋白的淋巴细胞、单核细胞和粒细胞的百分比发生了显著变化。