Haegert D G
Immunology. 1979 Nov;38(3):459-65.
Various preparations of human peripheral blood lymphocytes were examined for the presence of membrane-incorporated immunoglobulin (Ig) molecules using the direct antiglobulin rosetting reaction (DARR) and/or the mixed antiglobulin rosetting reaction (MARR). In an initial study, 97% of lymphocytes depleted of sheep erythrocyte (E) rosette-forming cells were found to be Ig positive by the MARR. This suggests that the majority of E rosette-negative lymphocytes are Ig-bearing B lymphocytes. Since most investigators divide E rosette-negative lymphocytes into an Ig-positive or B-cell population and an Ig-negative or null-cell population, it was decided to study further a well defined null-cell subset. Lymphocytes with membrane-labile IgG markers or L lymphocytes are reported to be E rosette negative, Ig negative by direct immunofluorescence (DIF), C3 receptor negative and Fc receptor positive. L lymphocytes were separated from DIF-positive B cells then tested for the presence of surface Ig, approximately 80% of L lymphocytes were found to be Ig positive with both the MARR and the DARR. Comparison of gamma, alpha and mu heavy chain determinants on both DIF-positive and L lymphocytes indicated that Ig isotypes are similar on both cell types. These various findings suggest that most L lymphocytes are of B-cell lineage.
运用直接抗球蛋白玫瑰花结反应（DARR）和/或混合抗球蛋白玫瑰花结反应（MARR），检测了人外周血淋巴细胞的各种制剂中是否存在膜结合免疫球蛋白（Ig）分子。在一项初步研究中，发现97%去除绵羊红细胞（E）玫瑰花结形成细胞的淋巴细胞通过MARR呈Ig阳性。这表明大多数E玫瑰花结阴性淋巴细胞是携带Ig的B淋巴细胞。由于大多数研究者将E玫瑰花结阴性淋巴细胞分为Ig阳性或B细胞群体以及Ig阴性或裸细胞群体，因此决定进一步研究一个明确界定的裸细胞亚群。据报道，具有膜不稳定IgG标记的淋巴细胞或L淋巴细胞E玫瑰花结阴性，直接免疫荧光（DIF）显示Ig阴性，C3受体阴性且Fc受体阳性。将L淋巴细胞与DIF阳性B细胞分离，然后检测表面Ig的存在，发现约80%的L淋巴细胞通过MARR和DARR均呈Ig阳性。对DIF阳性和L淋巴细胞上γ、α和μ重链决定簇的比较表明，两种细胞类型上的Ig同种型相似。这些不同的发现表明，大多数L淋巴细胞属于B细胞谱系。
