混合抗球蛋白玫瑰花结反应（MARR）和直接抗球蛋白玫瑰花结反应（DARR）：检测携带免疫球蛋白的B淋巴细胞的敏感试验。

The mixed antiglobulin rosetting reaction (MARR) and the direct antiglobulin rosetting reaction (DARR): sensitive tests for demonstration of immunoglobulin-bearing B lymphocytes.

作者信息

Haegert D G

出版信息

J Immunol Methods. 1981;41(1):1-8. doi: 10.1016/0022-1759(81)90268-4.

DOI:10.1016/0022-1759(81)90268-4

Abstract

The technology of the mixed antiglobulin rosetting (MARR) and the direct antiglobulin rosetting reaction (DARR) is reviewed. These tests have been found to be more sensitive than the standard direct immunofluorescence (DIF) method for delineation of Ig-bearing B lymphocytes and to have practical advantages over DIF. Further, with these rosette tests, an important B lymphocyte population has been identified which is Ig-negative by DIF and functionally quite different from typical DIF-positive B lymphocytes but which expresses a low density of surface membrane Ig that is detectable by the MARR and the DARR.

摘要

对混合抗球蛋白玫瑰花结试验（MARR）和直接抗球蛋白玫瑰花结反应（DARR）技术进行了综述。已发现这些试验在鉴定携带免疫球蛋白（Ig）的B淋巴细胞方面比标准直接免疫荧光法（DIF）更敏感，并且相对于DIF具有实际优势。此外，通过这些玫瑰花结试验，已鉴定出一个重要的B淋巴细胞群体，该群体通过DIF检测为Ig阴性，在功能上与典型的DIF阳性B淋巴细胞有很大不同，但表达低密度的表面膜Ig，可通过MARR和DARR检测到。

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