Si Yuan-Cheng, Miao Wei-Na, He Jia-Yue, Chen Li, Wang Yi-Li, Ding Wei-Jun
† Acupuncture and Tuina College, Guiyang College of Traditional Chinese Medicine, Dongqing South Road, Huaxi District, Guiyang 550025, P. R. China.
* Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Liutai Road 1166, Wenjiang District, Chengdu 610037, P. R. China.
Am J Chin Med. 2018 Oct 4:1-17. doi: 10.1142/S0192415X18500763.
Recently, gut flora has been linked to the onset of obesity and has been shown to influence the host's metabolism. Acupuncture is a well-known agent used for the treatment of numerous diseases such as obesity. This study aimed to explore the impacts of electroacupuncture treatment on gut microbiota composition and function in obese mice. Pyrosequencing of 16S rRNA genes and Metagenomic analysis of the fecal microbiota were used for this purpose. The basic parameters of body weight, Lee's index, serum lipid and epididymal adipose weight were ameliorated significantly after introducing an electroacupuncture intervention. Acidobacteria, Cyanobacteria and Basidiomycota (Normal group) and Fusobacteria, Firmicutes and Spirochmycetes (Model group) were remarkably affluent at the phylum level. Bacteroides sp. CAG: 927 and Prevotella sp. CAG: 1031 (Normal group), Lachnospiraceae bacterium and Helicobacter rodentium (Model group) at the species level were distinctly enriched. The structures and functions of the intestinal flora were significantly different between healthy and obese mice, and animals in the acupuncture group gradually tended towards healthy controls. Moreover, electroacupuncture altered the bacterial diversity and metabolic genes to establish new balance, observed the obvious change from 7[Formula: see text]d and stabilized gradually through 21[Formula: see text]d. These findings suggested gut flora could be a novel target of electroacupuncture treatment against obesity.
最近，肠道菌群已被证明与肥胖症的发生有关，并对宿主的新陈代谢产生影响。针灸是一种治疗多种疾病（如肥胖症）的常用手段。本研究旨在探讨电针治疗对肥胖小鼠肠道微生物群组成和功能的影响。为此采用了16S rRNA基因焦磷酸测序和粪便微生物群的宏基因组分析。引入电针干预后，体重、李氏指数、血脂和附睾脂肪重量等基本参数均有显著改善。在门水平上，酸杆菌门、蓝细菌门和担子菌门（正常组）以及梭杆菌门、厚壁菌门和螺旋体门（模型组）显著富集。在种水平上，拟杆菌属CAG:927和普雷沃菌属CAG:1031（正常组）、毛螺菌科细菌和啮齿柠檬酸螺旋杆菌（模型组）明显富集。健康小鼠和肥胖小鼠的肠道菌群结构和功能存在显著差异，针灸组的动物逐渐趋向于健康对照组。此外，电针改变了细菌多样性和代谢基因以建立新的平衡，在第7天观察到明显变化，并在21天内逐渐稳定。这些发现表明肠道菌群可能是电针治疗肥胖症的一个新靶点。
