Huang Wanyi, Yau Yuenming, Zhu Jingru, Wang Yingjie, Dai Zhipeng, Gan Huijuan, Qian Linchao, Yang Zongbao
School of Medicine, Xiamen University, Xiamen, China.
College of Acupuncture and Moxibustion, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Front Genet. 2022 Feb 23;13:824739. doi: 10.3389/fgene.2022.824739. eCollection 2022.
Electroacupuncture is a common treatment for chronic atrophic gastritis (CAG) in China. We aimed to determine the effects of electroacupuncture at zusanli (ST36) on intestinal microbiota in CAG rats. In total, 42 SD rats were randomly divided into normal (NC, 10 rats) and model (MG, 32 rats) groups. Rats in the MG group were established as CAG disease models. After that, the rats in the MG group were randomly divided into CAG (10 rats), electroacupuncture (EA, 10 rats), and Vitacoenzyme (Vit, 10 rats) groups. Rats in the NC and CAG groups were subjected to a 30-min/d confinement for 4 weeks. Rats in the EA group were given electroacupuncture at zusanli for 30 min/d for 4 weeks. Rats in the Vit group were given Vitacoenzyme solution 10 ml/(kg d) for 4 weeks. Histopathological changes in the gastric mucosa were observed with hematoxylin and eosin staining, and the gene expression level of p53, Bcl-2, and c-myc was determined using the qPCR method. The 16S rDNA sequencing technique was used to determine structural changes and relative abundance expression of intestinal flora. Compared with the NC group, gastric mucosal pathology in the CAG group revealed significant inflammatory infiltration, and the gastric mucosal lesions in the electroacupuncture group were improved remarkably; the expression of p53 and c-myc genes in the CAG group increased ( < 0.05), while the expression of Bcl-2 genes decreased ( < 0.05) in the EA group, that of p53 and c-myc genes decreased ( < 0.05), and that of Bcl-2 genes increased ( < 0.05). The abundance of bacteria such as , , and in the CAG group increased ( < 0.05), while that of bacteria such as , , and decreased ( < 0.05). The relative abundance of and in the EA group decreased ( < 0.05), while that of probiotic bacteria such as , , and Christensenellaceae increased ( < 0.05). Electroacupuncture at zusanli can promote the repair of pathological damage to the gastric mucosa in rats with CAG, and the mechanism might relate to the reduction in the relative abundance of harmful bacteria, increase in the relative abundance of intestinal probiotics, and regulation of the intestinal microbiota.
在中国，电针是治疗慢性萎缩性胃炎（CAG）的常用方法。我们旨在确定足三里（ST36）电针治疗对CAG大鼠肠道微生物群的影响。总共42只SD大鼠被随机分为正常组（NC，10只大鼠）和模型组（MG，32只大鼠）。MG组大鼠被建立为CAG疾病模型。之后，MG组大鼠被随机分为CAG组（10只大鼠）、电针组（EA，10只大鼠）和复合维生素组（Vit，10只大鼠）。NC组和CAG组大鼠每天被限制活动30分钟，持续4周。EA组大鼠每天接受足三里电针治疗30分钟，持续4周。Vit组大鼠每天给予复合维生素溶液10 ml/(kg·d)，持续4周。用苏木精-伊红染色观察胃黏膜的组织病理学变化，采用qPCR方法测定p53、Bcl-2和c-myc的基因表达水平。采用16S rDNA测序技术测定肠道菌群的结构变化和相对丰度表达。与NC组相比，CAG组胃黏膜病理显示有明显的炎症浸润，电针组胃黏膜病变明显改善；CAG组p53和c-myc基因表达增加（P<0.05），而EA组Bcl-2基因表达降低（P<0.05），电针组p53和c-myc基因表达降低（P<0.05），Bcl-2基因表达增加（P<0.05）。CAG组中如[未提及具体细菌名称1]、[未提及具体细菌名称2]和[未提及具体细菌名称3]等细菌的丰度增加（P<0.05），而如[未提及具体细菌名称4]、[未提及具体细菌名称5]和[未提及具体细菌名称6]等细菌的丰度降低（P<0.05）。EA组中[未提及具体细菌名称7]和[未提及具体细菌名称8]的相对丰度降低（P<0.05），而如[未提及具体细菌名称9]、[未提及具体细菌名称10]和科氏杆菌属等益生菌的相对丰度增加（P<0.05）。足三里电针可促进CAG大鼠胃黏膜病理损伤的修复，其机制可能与有害细菌相对丰度的降低、肠道益生菌相对丰度的增加以及肠道微生物群的调节有关。
