工程化具有微尺度血管化的脂肪组织，使其对脂活性激素的灌注产生响应。
Engineering of microscale vascularized fat that responds to perfusion with lipoactive hormones.
机构信息
Department of Biomedical Engineering, Boston University, Boston, MA 02215, United States of America.
出版信息
Biofabrication. 2018 Oct 30;11(1):014101. doi: 10.1088/1758-5090/aae5fe.
Abstract
Current methods to treat large soft-tissue defects mainly rely on autologous transfer of adipocutaneous flaps, a method that is often limited by donor site availability. Engineered vascularized adipose tissues can potentially be a viable and readily accessible substitute to autologous flaps. In this study, we engineered a small-scale adipose tissue with pre-patterned vasculature that enables immediate perfusion. Vessels formed after one day of perfusion and displayed barrier function after three days of perfusion. Under constant perfusion, adipose tissues remained viable and responded to lipoactive hormones insulin and epinephrine with lipid accumulation and loss, respectively. Adipocyte growth correlated inversely with distance away from the feeding vessel, as predicted by a Krogh-type model.
摘要
目前治疗大面积软组织缺损的方法主要依赖于自体脂肪皮瓣移植，但这种方法常常受到供区的限制。工程化血管化脂肪组织可能是自体皮瓣的一种可行且易于获得的替代品。在这项研究中，我们构建了具有预先设计的脉管系统的小规模脂肪组织，使其能够立即进行灌注。在灌注一天后形成血管，并且在灌注三天后显示出屏障功能。在持续灌注下，脂肪组织保持存活，并分别对脂解激素胰岛素和肾上腺素产生脂质积累和损失的反应。脂肪细胞的生长与远离供养血管的距离呈负相关，这与 Krogh 型模型的预测一致。
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本文引用的文献
1
Contribution of Adipose Tissue to Development of Cancer.脂肪组织对癌症发展的贡献。
Compr Physiol. 2017 Dec 12;8(1):237-282. doi: 10.1002/cphy.c170008.
2
Generation, Endothelialization, and Microsurgical Suture Anastomosis of Strong 1-mm-Diameter Collagen Tubes.直径1毫米的高强度胶原管的制备、内皮化及显微外科缝合吻合术
Tissue Eng Part A. 2017 Apr;23(7-8):335-344. doi: 10.1089/ten.TEA.2016.0339. Epub 2017 Jan 13.
3
The Use of Silk as a Scaffold for Mature, Sustainable Unilocular Adipose 3D Tissue Engineered Systems.丝绸作为成熟、可持续的单房脂肪三维组织工程系统支架的应用。
Adv Healthc Mater. 2016 Jul;5(13):1667-77. doi: 10.1002/adhm.201600211. Epub 2016 May 19.
4
Dissecting adipose tissue lipolysis: molecular regulation and implications for metabolic disease.解析脂肪组织脂解：分子调控及其对代谢性疾病的影响。
J Mol Endocrinol. 2014 Jun;52(3):R199-222. doi: 10.1530/JME-13-0277. Epub 2014 Feb 27.
5
Crosslinking of collagen scaffolds promotes blood and lymphatic vascular stability.胶原蛋白支架的交联促进血液和淋巴管的稳定性。
J Biomed Mater Res A. 2014 Sep;102(9):3186-3195. doi: 10.1002/jbm.a.34990. Epub 2013 Oct 22.
6
The mechanical properties of human adipose tissues and their relationships to the structure and composition of the extracellular matrix.人体脂肪组织的机械性能及其与细胞外基质结构和组成的关系。
Am J Physiol Endocrinol Metab. 2013 Dec;305(12):E1427-35. doi: 10.1152/ajpendo.00111.2013. Epub 2013 Oct 8.
7
Determination of vascular permeability coefficients under slow luminal filling.在缓慢管腔填充下测定血管通透性系数。
Microvasc Res. 2013 Nov;90:117-20. doi: 10.1016/j.mvr.2013.07.003. Epub 2013 Jul 23.
8
Protocol for effective differentiation of 3T3-L1 cells to adipocytes.3T3-L1 细胞向脂肪细胞有效分化的方案。
Anal Biochem. 2012 Jun 1;425(1):88-90. doi: 10.1016/j.ab.2012.03.005. Epub 2012 Mar 13.
9
Lipolytic function of adipocyte/endothelial cocultures.脂肪细胞/内皮细胞共培养物的脂肪分解功能。
Tissue Eng Part A. 2011 May;17(9-10):1437-44. doi: 10.1089/ten.TEA.2010.0527. Epub 2011 Mar 3.
10
Methods for forming human microvascular tubes in vitro and measuring their macromolecular permeability.体外形成人微血管管及其大分子通透性测量方法。
Methods Mol Biol. 2011;671:281-93. doi: 10.1007/978-1-59745-551-0_17.
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