Famele Marco, Mancinelli Rosanna, Ferranti Carolina, Zoratto Francesca, Thomas Felia, Romano Emilia, Laviola Giovanni, Adriani Walter, Draisci Rosa
Centro Nazionale Sostanze Chimiche, Prodotti Cosmetici e Protezione del Consumatore, Istituto Superiore di Sanità, Rome, Italy.
Centro di Riferimento Scienze Comportamentali e Salute Mentale, Istituto Superiore di Sanità, Rome, Italy.
Ann Ist Super Sanita. 2018 Jul-Sep;54(3):176-184. doi: 10.4415/ANN_18_03_03.
This study investigates the transfer of nicotine from lactating dams to their offspring through breast milk, in the frame of a research focused to ascertain toxicological and neuro-behavioural effects on pups as consequence of either unavoidable ("yoked & forced") or voluntary ("freely-chosen") maternal nicotine exposure. To this aim, plasmatic concentrations of nicotine and cotinine were determined by LC-MS/MS in Wistar rat pups whose mothers were orally administered with nicotine during lactation. Mothers were divided into a voluntary drinking group, an unavoidable consumption group, and controls. The limits of detection and quantification of the LC-MS/MS method were 0.20 and 0.65 ng/mL, respectively. Within-laboratory reproducibility (CV%) was <12%, with recovery of 86.2-118.8%. Results showed the presence of nicotine in 67% of samples from freely-chosen consumption group (1.30 ± 0.31 ng/mL) and in 60% of samples from yoked-consumption group (1.19 ± 0.62 ng/mL); cotinine was found in all the samples from freely-chosen (1.92 ± 0.77 ng/mL) and yoked-consumption groups (1.43 ± 0.30 ng/mL). Data provide an evidence-based support to maternal/offspring nicotine transfer as function of different ways of oral exposure.
本研究在一项旨在确定母体尼古丁暴露（无论是不可避免的“被动与强制”暴露还是自愿的“自由选择”暴露）对幼崽产生的毒理学和神经行为学影响的研究框架内，调查了哺乳期母鼠通过母乳向其后代转移尼古丁的情况。为此，采用液相色谱-串联质谱法（LC-MS/MS）测定了哺乳期经口服给予尼古丁的Wistar大鼠幼崽血浆中尼古丁和可替宁的浓度。母鼠被分为自愿饮用组、不可避免摄入组和对照组。LC-MS/MS方法的检测限和定量限分别为0.20 ng/mL和0.65 ng/mL。实验室内重现性（CV%）<12%，回收率为86.2 - 118.8%。结果显示，自由选择摄入组67%的样本中存在尼古丁（1.30±0.31 ng/mL），被动摄入组60%的样本中存在尼古丁（1.19±0.62 ng/mL）；自由选择摄入组和被动摄入组的所有样本中均检测到可替宁（自由选择摄入组为1.92±0.77 ng/mL，被动摄入组为1.43±0.30 ng/mL）。这些数据为不同口服暴露方式下母体/后代尼古丁转移提供了基于证据的支持。
