Shapleigh J P, Davies K J, Payne W J
Biochim Biophys Acta. 1987 Feb 25;911(3):334-40. doi: 10.1016/0167-4838(87)90074-4.
Gas chromatography revealed that exposure of extracts of the denitrifiers 'Achromobacter cycloclastes', Paracoccus denitrificans, Pseudomonas aeruginosa and Pseudomonas perfectomarina to Triton X-100 inhibited reduction of NO to N2O, and thus concomitantly inhibited reduction of NO2- to N2O. After exposure of extracts to Triton X-100, the ratio of H+ consumed to NO2- added decreased from approx. 2.0 (for untreated extracts) to approx. 1.5, which indicated that NO2- was reduced to NO by the treated extracts. Addition of a CHAPS-soluble extract (devoid of nitrite reductase activity but rich in nitric-oxide reductase activity) to the Triton X-100-treated extract of P. denitrificans restored capacity for reduction of NO2- on to N2O. Exposure to either the NO that accumulated from reduction of NO2- or to enthetic NO transiently inhibited rates of NO2- reduction in Triton X-100-treated extracts. Use of an Oxides of Nitrogen analyzer indicated that only 5-33% of NO2- reduced by untreated extracts appeared in the stripping gas as NO, whereas 80-95% of NO2- reduced by Triton X-100-treated extracts was recovered as NO.
气相色谱分析表明，反硝化菌“环裂无色杆菌”、脱氮副球菌、铜绿假单胞菌和完美假单胞菌的提取物暴露于Triton X-100后，一氧化氮还原为一氧化二氮的过程受到抑制，因此亚硝酸根还原为一氧化二氮的过程也随之受到抑制。提取物暴露于Triton X-100后，消耗的氢离子与添加的亚硝酸根的比例从约2.0（未处理的提取物）降至约1.5，这表明经处理的提取物将亚硝酸根还原为了一氧化氮。向经Triton X-100处理的脱氮副球菌提取物中添加一种可溶于CHAPS的提取物（缺乏亚硝酸还原酶活性但富含一氧化氮还原酶活性），恢复了将亚硝酸根还原为一氧化二氮的能力。暴露于由亚硝酸根还原积累的一氧化氮或外源性一氧化氮中，会暂时抑制经Triton X-100处理的提取物中亚硝酸根的还原速率。使用氮氧化物分析仪表明，未处理的提取物还原的亚硝酸根中只有5-33%以一氧化氮的形式出现在吹扫气中，而经Triton X-100处理的提取物还原的亚硝酸根中有80-95%以一氧化氮的形式回收。
