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人类特异性STR系统SureID 21G和SureID 23comp（健康基因技术公司）在黑猩猩（Pan Troglodytes）中的跨物种验证。

Cross-species validation of human specific STR system, SureID 21G and SureID 23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes).

作者信息

Singh Abhishek, Thakur Mukesh, Sahajpal Vivek, Singh Sujeet K, Chandra Kailash, Sharma Arun, Devi Nisha, Bernot Ausma

机构信息

Zoological Survey of India, New Alipore, Kolkata, West Bengal, 700 053, India.

State Forensic Science Laboratory, Directorate of Forensics Services, Junga, Shimla, Himachal Pradesh, 171218, India.

出版信息

BMC Res Notes. 2019 Nov 19;12(1):750. doi: 10.1186/s13104-019-4801-3.

DOI:10.1186/s13104-019-4801-3
PMID:31744541
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6862757/
Abstract

OBJECTIVES

The human specific commercially available STRs system are often not tested in non human primates for their cross applicability. The aim of this study is to test Cross-species validation of two commercially available human specific STR kits i.e. SureID 21G and SureID 23comp (Health Gene Technologies) for their positive application in Chimpanzee (Pan troglodytes).

RESULT

In SureID 21G, 19 loci amplified and while 20 loci amplified in SureID 23comp. All the amplified loci in both STR kits were found polymorphic and the locus Amelogenin showed differential banding patterns between male and female revealing their known gender. The present study validates the applicability of these human specific STR kits in Chimpanzee that can be used in forensics analysis, paternity testing and population genetic studies.

摘要

目的

市售的人类特异性短串联重复序列（STR）系统通常未在非人类灵长类动物中测试其交叉适用性。本研究的目的是测试两种市售的人类特异性STR试剂盒，即SureID 21G和SureID 23comp（健康基因技术公司）在黑猩猩（Pan troglodytes）中的阳性应用的种间验证。

结果

在SureID 21G中，19个基因座扩增，而在SureID 23comp中有20个基因座扩增。两种STR试剂盒中所有扩增的基因座均为多态性，牙釉蛋白基因座在雄性和雌性之间显示出不同的条带模式，揭示了它们已知的性别。本研究验证了这些人类特异性STR试剂盒在黑猩猩中的适用性，可用于法医分析、亲子鉴定和群体遗传学研究。

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