Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan.
Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
PLoS One. 2018 Oct 4;13(10):e0204866. doi: 10.1371/journal.pone.0204866. eCollection 2018.
PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) regulates components of the cell cycle, including cell growth, immune responses, DNA damage repair, apoptosis, and inflammation. PBK/TOPK may also accelerate tumorigenesis in colorectal cancer.
We investigated the impact of PBK/TOPK on the clinical outcome of colorectal cancer patients to further identify its role in colorectal cancer. PBK/TOPK immunoreactivity was analyzed by immunohistochemistry in 162 cancer specimens from primary colorectal cancer patients.
The mean follow-up time after surgery was 5.4 years (medium: 3.9 years; range 0.01 to 13.1 years). The prognostic value of PBK/TOPK on overall survival was determined by Kaplan-Meier analysis and Cox proportional hazard models. PBK/TOPK was expressed in both the cytoplasm and nucleus. High PBK/TOPK expression in tumor cells was significantly associated with advanced T value. The 5-year survival rate was greater for patients with high total PBK/TOPK expression than with low PBK/TOPK expression (58.3% vs 34.4%, P = 0.005). Multivariate analyses showed that low-scoring cytoplasmic PBK/TOPK, negative nuclear PBK/TOPK, low total PBK/TOPK, and advanced tumor stage were correlated with poor overall patient survival.
We suggest that PBK/TOPK expression, detected by IHC staining, could be used as an independent prognostic marker for colorectal cancer patients.
PDZ 结合激酶/T-LAK 细胞起源的蛋白激酶（PBK/TOPK）调节细胞周期的组成部分，包括细胞生长、免疫反应、DNA 损伤修复、细胞凋亡和炎症。PBK/TOPK 还可能加速结直肠癌的肿瘤发生。
我们通过免疫组织化学分析 162 例原发性结直肠癌患者的癌症标本，研究了 PBK/TOPK 对结直肠癌患者临床结局的影响，以进一步确定其在结直肠癌中的作用。
手术后的平均随访时间为 5.4 年（中位数：3.9 年；范围 0.01 至 13.1 年）。通过 Kaplan-Meier 分析和 Cox 比例风险模型确定 PBK/TOPK 对总生存的预后价值。PBK/TOPK 在肿瘤细胞中的表达既存在于细胞质中，也存在于细胞核中。肿瘤细胞中高 PBK/TOPK 表达与较高的 T 分期显著相关。高总 PBK/TOPK 表达的患者 5 年生存率高于低 PBK/TOPK 表达的患者（58.3%比 34.4%，P=0.005）。多变量分析显示，细胞质中低评分的 PBK/TOPK、核阴性的 PBK/TOPK、低总 PBK/TOPK 和肿瘤分期较晚与患者总体生存不良相关。
我们认为，通过免疫组化染色检测到的 PBK/TOPK 表达可作为结直肠癌患者的独立预后标志物。
